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1. (WO2017152122) AUTOMATED NESTED RECOMBINASE POLYMERASE AMPLIFICATION

Pub. No.:    WO/2017/152122    International Application No.:    PCT/US2017/020782
Publication Date: Sat Sep 09 01:59:59 CEST 2017 International Filing Date: Sat Mar 04 00:59:59 CET 2017
IPC: C12Q 1/68
C12P 19/34
C12N 9/16
Applicants: ALERE SAN DIEGO INC.
Inventors: WHYTE, Murray John
ARMES, Niall A.
PIEPENBURG, Olaf
NENTWICH, Oliver
GREENWOOD, Catherine Jean
Title: AUTOMATED NESTED RECOMBINASE POLYMERASE AMPLIFICATION
Abstract:
A flu assay system including a sample module, a microfluidic nucleic acid amplification device, and an analyzer to facilitate fully automated nested recombinase polymerase amplification (RPA) on a sample delivered to the nucleic acid amplification device via the sample module. The assay includes providing a sample to a microfluidic device, and amplifying a target polynucleotide sequence in the sample. Amplifying the target polynucleotide sequence includes performing a first round of amplification on the sample to yield a first amplification product, and performing a second round of amplification on the first amplification product to yield a second amplification product. The second amplification product includes a smaller sequence completely contained within the first amplification product produced during the first round of amplification.