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1. (WO2017152073) METHODS AND COMPOSITIONS FOR EX VIVO EXPANSION OF VERY SMALL EMBRYONIC-LIKE STEM CELLS (VSELS)
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Pub. No.: WO/2017/152073 International Application No.: PCT/US2017/020696
Publication Date: 08.09.2017 International Filing Date: 03.03.2017
IPC:
C12N 5/0735 (2010.01) ,A61K 35/12 (2015.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
073
Embryonic cells or tissues; Foetal cells or tissues
0735
Embryonic stem cells; Embryonic germ cells
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
Applicants:
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION, INC. [US/US]; 300 East Market Street Suite 300 Louisville, KY 40202, US
Inventors:
RATAJCZAK, Mariusz Z; US
RATAJCZAK, Janina; US
KUCIA, Magdalena; US
MILLER, Donald; US
Agent:
PERKINS, Christopher, P.; US
Priority Data:
62/303,88804.03.2016US
Title (EN) METHODS AND COMPOSITIONS FOR EX VIVO EXPANSION OF VERY SMALL EMBRYONIC-LIKE STEM CELLS (VSELS)
(FR) PROCÉDÉS ET COMPOSITIONS POUR L'EXPANSION EX VIVO DE TRÈS PETITES CELLULES SOUCHES DE TYPE EMBRYONNAIRE (VSEL)
Abstract:
(EN) Methods for ex vivo expansion of very small embryonic like stem cells in the absence of feeder cells are provided. In some embodiments the methods include providing a plurality of VSELs; and growing the VSELs in a culture medium that includes a histone deacetylase inhibitor, luteinizing hormone, follicle-stimulating hormone, and optionally transforming growth factor beta in an amount that is sufficient to overcome quiescence of the VSELs. Also provided are feeder cell-free cell cultures, ex vivo expanded VSELs, pharmaceutical compositions that include the disclosed ex vivo expanded VSELs, methods for overcoming quiescence in VSELs, methods for re-establishing imprinting in VSELs, method for treating injuries to tissues in subjects, methods for repopulating cell types in subjects, methods for bone marrow transplantation, methods for treating radiation exposure in subjects, and methods that relate to regenerative medicine.
(FR) La présente invention concerne des procédés pour l'expansion ex vivo de très petites cellules souches de type embryonnaire en l'absence de cellules nourricières. Dans certains modes de réalisation, les procédés consistent à utiliser une pluralité de VSEL ; et à cultiver les VSEL dans un milieu de culture qui comprend un inhibiteur d'histone désacétylase, une hormone lutéinisante, une hormone folliculostimulante et éventuellement un facteur de croissance transformant bêta en une proportion suffisante pour surmonter la quiescence des VSEL. L'invention concerne également des cultures de cellules exemptes de cellules nourricières, des VSEL expansées ex vivo, des compositions pharmaceutiques comprenant les VSEL expansées ex vivo décrites, des procédés permettant de surmonter la quiescence des VSEL, des procédés permettant de rétablir l'empreinte dans les VSEL, une méthode de traitement de lésions tissulaires chez des sujets, des procédés permettant de repeupler des types de cellules chez des sujets, des méthodes de transplantation de moelle osseuse, des méthodes de traitement par exposition à des rayonnements chez des sujets et des méthodes relatives à la médecine régénérative.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)