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1. (WO2017151059) MULTIPLEXABLE ACTIVATION OF SILENT BIOSYNTHETIC CLUSTERS IN NATIVE ACTINOMYCETE HOSTS FOR NATURAL PRODUCT DISCOVERY
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Pub. No.: WO/2017/151059 International Application No.: PCT/SG2017/050092
Publication Date: 08.09.2017 International Filing Date: 28.02.2017
IPC:
C12N 15/76 (2006.01) ,C12N 9/22 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
76
for Actinomyces; for Streptomyces
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
22
Ribonucleases
Applicants:
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632, SG
Inventors:
ZHANG, Mingzi; SG
WONG, Fong Tian; SG
ZHAO, Huimin; SG
Agent:
SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531 Robinson Road Post Office Singapore 903031, SG
Priority Data:
10201601523W29.02.2016SG
Title (EN) MULTIPLEXABLE ACTIVATION OF SILENT BIOSYNTHETIC CLUSTERS IN NATIVE ACTINOMYCETE HOSTS FOR NATURAL PRODUCT DISCOVERY
(FR) ACTIVATION MULTIPLEXABLE DE GROUPES BIOSYNTHÉTIQUES SILENCIEUX DANS DES HÔTES ACTINOMYCÈTES NATIFS POUR LA DÉCOUVERTE DE PRODUITS NATURELS
Abstract:
(EN) Disclosed herein are recombinant methods of activating expression of one or more biosynthetic gene cluster(s), or one or more target gene(s) in a biosynthetic gene cluster comprising more than one gene, the method comprising inserting one or more promoter(s) using CRISPR technology at one or more transcriptionally functional location(s) relative to the biosynthetic gene cluster(s) or the target gene(s) in the biosynthetic gene cluster(s), whereby the insertion of the promoter(s) results in increased expression of the biosynthetic gene cluster(s) or target gene(s) compared to the expression level of an unmodified biosynthetic gene cluster(s) or target gene(s). Also disclosed herein are recombinant expression plasmids for activating expression of a biosynthetic gene cluster(s).
(FR) L'invention concerne des procédés par recombinaison d'activation de l'expression d'au moins un groupe de gènes biosynthétiques, ou d'au moins un gène cible dans un groupe de gènes biosynthétiques comprenant plus d'un gène, le procédé consistant à insérer un ou plusieurs promoteurs à l'aide de la technologie CRISPR dans au moins un emplacement fonctionnel sur le plan transcriptionnel par rapport au(x) groupe(s) de gènes biosynthétiques ou du ou des gènes cibles dans le groupe de gènes biosynthétiques, l'introduction du ou des promoteurs entraînant une augmentation de l'expression du groupe ou des groupes de gènes biosynthétiques ou du ou des gènes cibles par comparaison avec le niveau d'expression d'un ou plusieurs groupes de gènes biosynthétiques ou d'un ou plusieurs gènes cibles non modifiés. L'invention concerne également des plasmides d'expression recombinés pour activer l'expression d'un ou plusieurs groupes de gènes biosynthétiques.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)