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1. (WO2017150916) COMPOSITION FOR SOLUBILIZATION OF RECOMBINANT PROTEINS, METHOD THEREFOR, AND METHOD FOR PRODUCING RECOMBINANT ANTIGEN BY USING SAME
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/150916 International Application No.: PCT/KR2017/002273
Publication Date: 08.09.2017 International Filing Date: 02.03.2017
IPC:
C07K 1/113 (2006.01) ,C07K 14/005 (2006.01) ,G01N 33/569 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
107
by chemical modification of precursor peptides
113
without change of the primary structure
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005
from viruses
01
DNA viruses
03
Herpetoviridae, e.g. pseudorabies virus
05
Epstein-Barr virus
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
569
for micro-organisms, e.g. protozoa, bacteria, viruses
Applicants:
에치비아이주식회사 HBI CO., LTD. [KR/KR]; 경기도 안양시 동안구 벌말로 140, 7508, 7204(관양동, 동일테크노타운7차) (Dongil Techno Town 7 Cha, Gwanyang-dong)7204, 7508, 140, Beolmal-ro Dongan-gu, Anyang-si Gyeonggi-do 14057, KR
Inventors:
김수옥 KIM, Soo Ok; KR
김석준 KIM, Suk Joon; KR
임지혜 LIM, Ji Hye; KR
장선옥 JANG, Sun Ok; KR
김정옥 KIM, Jung Ok; KR
이승화 LEE, Seong Hwa; KR
홍선표 HONG, Sun Pyo; KR
Agent:
구현서 KOO, Hyun-Seo; 서울시 강남구 강남대로66길 8, 9층 9F, 8, Gangnam-daero 66-gil Gangnam-gu, Seoul 06253, KR
Priority Data:
10-2016-002643404.03.2016KR
Title (EN) COMPOSITION FOR SOLUBILIZATION OF RECOMBINANT PROTEINS, METHOD THEREFOR, AND METHOD FOR PRODUCING RECOMBINANT ANTIGEN BY USING SAME
(FR) COMPOSITION DE SOLUBILISATION DE PROTÉINES RECOMBINÉES, PROCÉDÉ ASSOCIÉ ET PROCÉDÉ DE PRODUCTION D'ANTIGÈNE RECOMBINÉ L'UTILISANT
(KO) 재조합 단백질의 가용화를 위한 조성물, 그 방법 및 이를 이용한 재조합 항원의 생산 방법
Abstract:
(EN) The present invention relates to a recombinant protein solubilization composition, a solubilization method using the same, and an application thereof, the recombinant protein solubilization composition containing, as essential ingredients, a) a buffer material having a pH of 6.5-9.5, b) a mixture of glutathione (GSH) and oxidized glutathione (GSSG), and c) sodium chloride (NaCl), and optionally containing arginine or a mixture of magnesium chloride and calcium chloride. A protein to be expressed as an inclusion body can be more easily prepared and mass produced into a recombinant antigen through the solubilization composition of the present invention, and the prepared recombinant antigen can be applied, as a protein marker material, to an immunochromatographic kit for rapid diagnosis and the like.
(FR) La présente invention concerne une composition de solubilisation de protéines recombinées, un procédé de solubilisation l’utilisant et une application de cette dernière. La composition de solubilisation de protéines recombinées contient, comme ingrédients essentiels, a) un matériau tampon ayant un pH de 6,5 à 9,5, b) un mélange de glutathion (GSH) et de glutathion oxydé (GSSG), et c) du chlorure de sodium (NaCl), et contient éventuellement de l’arginine ou un mélange de chlorure de magnésium et de chlorure de calcium. Une protéine devant être exprimée sous forme de corps d'inclusion peut être préparée plus facilement et produite en masse en un antigène recombiné à l'aide de la composition de solubilisation de la présente invention, et l'antigène recombiné préparé peut être appliqué, en tant que matériau marqueur de protéine, à un kit immunochromatographique pour un diagnostic rapide et similaire.
(KO) 본 발명은 a)pH 6.5, 내지 9.5의 완충 물질;b)굴루타치온(GSH) 및 산화형 글루타치온(GSSG) 혼합물; 및 c)염화나트륨(NaCl)를 필수 구성성분으로 포함하며,선택적으로 아르기닌, 또는 염화 마그네슘 및 염화 칼슘 혼합물을 포함하는 재조합 단백질 가용화 조성물 및 이를 이용한 가용화 방법 및 그 응용에 관한 것이다. 본 발명의 가용화 조성물을 통해 내포체로 발현되는 단백질을 보다 손쉽게 재조합 항원으로 제조, 대량생산 할 수 있고, 이렇게 조제된 재조합 항원을 면역크로마토그래피 신속진단키트 등에 단백질 마커 원료로 적용 가능하다.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)