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1. (WO2017150294) METHOD FOR PRODUCING PLURIPOTENT STEM CELL-LIKE SPHEROID AND PLURIPOTENT STEM CELL-LIKE SPHEROID

Pub. No.:    WO/2017/150294    International Application No.:    PCT/JP2017/006546
Publication Date: Sat Sep 09 01:59:59 CEST 2017 International Filing Date: Thu Feb 23 00:59:59 CET 2017
IPC: C12N 5/071
C12M 3/00
Applicants: YAMAGUCHI Yoshitaka
山口 良考
Inventors: YAMAGUCHI Yoshitaka
山口 良考
Title: METHOD FOR PRODUCING PLURIPOTENT STEM CELL-LIKE SPHEROID AND PLURIPOTENT STEM CELL-LIKE SPHEROID
Abstract:
Provided are a method for producing a pluripotent stem cell-like spheroid and a pluripotent stem cell-like spheroid. Differentiated living cells are treated with an enzyme to give spherical cells. Then, the spherical cells are stationarily cultured at a cell density of 3×105-7×108/ml using a non-cell-adhesive cell culture container. Thus, the cells form a cell aggregation mass in a size corresponding to the cell count. The obtained cell aggregation mass (a spheroid) expresses a pluripotent stem cell marker and transforms into pluripotent stem cell-like cells.