Provided are a method for producing a pluripotent stem cell-like spheroid and a pluripotent stem cell-like spheroid. Differentiated living cells are treated with an enzyme to give spherical cells. Then, the spherical cells are stationarily cultured at a cell density of 3×105-7×108/ml using a non-cell-adhesive cell culture container. Thus, the cells form a cell aggregation mass in a size corresponding to the cell count. The obtained cell aggregation mass (a spheroid) expresses a pluripotent stem cell marker and transforms into pluripotent stem cell-like cells.