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1. (WO2017147945) SEQUENCING METHOD FOR GENETIC MAPPING OF DNA 5-METHYLCYTOSINE AND 5-HYDROXYMETHYLCYTOSINE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2017/147945 International Application No.: PCT/CN2016/075863
Publication Date: 08.09.2017 International Filing Date: 08.03.2016
IPC:
C12Q 1/68 (2006.01) ,C40B 50/06 (2006.01) ,C12N 15/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
50
Methods of creating libraries, e.g. combinatorial synthesis
06
Biochemical methods, e.g. using enzymes or whole viable micro-organisms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
上海易毕恩基因科技有限公司 SHANGHAI EPICAN GENETECH CO. LTD. [CN/CN]; 中国上海市 自由贸易试验区爱迪生路328号201-2室 Room 201-2 328 Aidisheng Road, China (Shanghai) Pilot Free Trade Zone Shanghai 201210, CN
Inventors:
陆星宇 LU, Xingyu; CN
宋艳群 SONG, Yanqun; CN
Agent:
上海精晟知识产权代理有限公司 SHANGHAI CPTO INTELLECTUAL PROPERTY AGENCY CO., LTD; 中国上海市 杨浦区控江路1555号A座2518室 Room 2518, Building A No.1555 Kong Jiang Road, Yangpu District Shanghai 200092, CN
Priority Data:
201610119096.202.03.2016CN
Title (EN) SEQUENCING METHOD FOR GENETIC MAPPING OF DNA 5-METHYLCYTOSINE AND 5-HYDROXYMETHYLCYTOSINE
(FR) PROCÉDÉ DE SÉQUENÇAGE POUR LA CARTOGRAPHIE GÉNÉTIQUE DE 5-MÉTHYLCYTOSINE ET DE 5-HYDROXYMÉTHYLCYTOSINE D'ADN
(ZH) DNA5-甲基胞嘧啶与5-羟甲基胞嘧啶基因图谱测序方法
Abstract:
(EN) Provided is a sequencing method for the genetic mapping of DNA 5-methylcytosine and 5-hydroxymethylcytosine. The method employs next generation sequencing and library construction technology for trace free DNA and a high-efficiency chemical bioorthogonal reaction, which greatly enhances the selectivity and efficiency of a solid-phase surface binding to a DNA modified base.
(FR) L'invention concerne un procédé de séquençage pour la cartographie génétique de 5-méthylcytosine et de 5-hydroxyméthylcytosine d'ADN. Le procédé utilise un séquençage de nouvelle génération et une technologie de construction de bibliothèque pour retrouver l'ADN libre à l'état de trace et une réaction bioorthogonale chimique à hauts efficacité, ce qui améliore considérablement la sélectivité et l'efficacité de liaison d'une surface en phase solide à une base modifiée de l'ADN.
(ZH) 本发明提供了一种DNA5-甲基胞嘧啶与5-羟甲基胞嘧啶基因图谱测序方法,所述方法采用微量游离DNA二代测序建库技术及高效的化学生物学正交反应,极大增强了固相表面与DNA修饰碱基结合的选择性和效率。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)