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1. (WO2017147729) METHOD FOR INSTRUMENT-FREE EXTRACTION OF TOTAL DNA FROM YEAST SAMPLE AFTER NUCLEIC ACID AMPLIFICATION

Pub. No.:    WO/2017/147729    International Application No.:    PCT/CN2016/000651
Publication Date: Sat Sep 09 01:59:59 CEST 2017 International Filing Date: Fri Nov 25 00:59:59 CET 2016
IPC: C12N 15/10
C12Q 1/68
Applicants: THE SECOND MILITARY MEDICAL UNIVERSITY
中国人民解放军第二军医大学
USTAR BIOTECHNOLOGIES(HANGZHOU) LTD.
杭州优思达生物技术有限公司
Inventors: CHEN, Min
陈敏
FANG, Wenjie
方文捷
PAN, Weihua
潘炜华
LIAO, Wanqing
廖万清
YOU, Qimin
尤其敏
HONG, Nan
洪南
LIU, Jia
刘加
SUN, Gang
孙刚
ZHANG, Lei
张蕾
LI, Yingfang
李颖芳
JIANG, Weiwei
姜伟伟
ZHAO, Haixia
赵海霞
LI, Juan
李娟
WU, Junqi
吴俊琪
YU, Shichong
俞世冲
ZHANG, Xinying
张辛颖
Title: METHOD FOR INSTRUMENT-FREE EXTRACTION OF TOTAL DNA FROM YEAST SAMPLE AFTER NUCLEIC ACID AMPLIFICATION
Abstract:
Provided is a method for instrument-free extraction of total DNA from an yeast sample after nucleic acid amplification. The method integrates a chemolysis method and an enzymolysis method, and uses a suction device, a filtering device, and a nucleic acid extraction syringe. The invention can complete a DNA extraction process between 1.5 hours to 3 hours without using any powered device.