WO2017144496 BETA-LACTAMASE VARIANTS

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Publication Number WO/2017/144496

Publication Date 31.08.2017

International Application No. PCT/EP2017/053986

International Filing Date 22.02.2017

IPC

CPC

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Applicants

DA VOLTERRA [FR]/[FR]
BIOASTER [FR]/[FR]

Inventors

DE GUNZBURG, Jean
DOCQUIER, Jean-Denis

Agents

CABINET BECKER ET ASSOCIES

Priority Data

16305209.5

23.02.2016

Publication Language English (en)

Filing Language English (EN)

Designated States

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Title

(EN) BETA-LACTAMASE VARIANTS

(FR) VARIANTES DE LA BÊTA-LACTAMASE

Abstract

(EN) The present invention relates to an isolated polypeptide having beta-lactamase activity and nucleic acid sequences encoding the polypeptide. The isolated polypeptide of the invention is a VIM-2 variant with improved properties such as improved protease stability, stability in intestinal medium, improved activity against one or more antibiotics, improved specific activity and/or improved production in a host cell.

(FR) La présente invention concerne un polypeptide isolé ayant une activité bêta-lactamase et des séquences d'acide nucléique codant le polypeptide. Le polypeptide isolé selon l'invention est une variante de VIM-2 ayant des propriétés améliorées, par exemple une plus grande stabilité de la protéase, une stabilité en milieu intestinal, une plus grande activité vis-à-vis d'un ou de plusieurs antibiotiques, une plus grande activité spécifique et/ou une production plus importante dans une cellule hôte.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14	Hydrolases (3.)
78	acting on carbon to nitrogen bonds other than peptide bonds (3.5)
86	acting on amide bonds in cyclic amides, e.g. penicillinase

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14	Hydrolases (3.)
78	acting on carbon to nitrogen bonds other than peptide bonds (3.5)
86	acting on amide bonds in cyclic amides, e.g. penicillinase

A	HUMAN NECESSITIES
61	MEDICAL OR VETERINARY SCIENCE; HYGIENE
K	PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31	Medicinal preparations containing organic active ingredients
33	Heterocyclic compounds
395	having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
41	having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
425	Thiazoles
429	condensed with heterocyclic ring systems
43	Compounds containing 4-thia-1-azabicyclo [3.2.0] heptane ring systems, i.e. compounds containing a ring system of the formula , e.g. penicillins, penems

A	HUMAN NECESSITIES
61	MEDICAL OR VETERINARY SCIENCE; HYGIENE
K	PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38	Medicinal preparations containing peptides

A	HUMAN NECESSITIES
61	MEDICAL OR VETERINARY SCIENCE; HYGIENE
K	PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38	Medicinal preparations containing peptides
16	Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
43	Enzymes; Proenzymes; Derivatives thereof
46	Hydrolases (3)
50	acting on carbon-nitrogen bonds, other than peptide bonds (3.5), e.g. asparaginase

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1. WO2017144496 - BETA-LACTAMASE VARIANTS

A	HUMAN NECESSITIES
61	MEDICAL OR VETERINARY SCIENCE; HYGIENE
P	SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
31	Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
04	Antibacterial agents

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Y	ENZYMES
305	Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
02	in cyclic amides (3.5.2)
02006	Beta-lactamase (3.5.2.6)