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1. (WO2017138484) METHOD FOR DETECTING TARGET NUCLEIC ACID AND NUCLEIC ACID PROBE USED THEREIN
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/138484 International Application No.: PCT/JP2017/004191
Publication Date: 17.08.2017 International Filing Date: 06.02.2017
IPC:
C12Q 1/68 (2006.01) ,C12N 15/09 (2006.01) ,G01N 21/78 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
75
Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77
by observing the effect on a chemical indicator
78
producing a change of colour
Applicants:
栄研化学株式会社 EIKEN KAGAKU KABUSHIKI KAISHA [JP/JP]; 東京都台東区台東4丁目19番9号 4-19-9, Taito, Taito-ku, Tokyo 1108408, JP
Inventors:
佐伯 遼平 SAEKI Ryohei; JP
Agent:
長谷川 芳樹 HASEGAWA Yoshiki; JP
清水 義憲 SHIMIZU Yoshinori; JP
木元 克輔 KIMOTO Kosuke; JP
Priority Data:
2016-02265909.02.2016JP
Title (EN) METHOD FOR DETECTING TARGET NUCLEIC ACID AND NUCLEIC ACID PROBE USED THEREIN
(FR) PROCÉDÉ DE DÉTECTION D'ACIDE NUCLÉIQUE CIBLE ET SONDE D'ACIDE NUCLÉIQUE UTILISÉE DANS CE PROCÉDÉ
(JA) 標的核酸を検出する方法およびそれに用いる核酸プローブ
Abstract:
(EN) The present invention is, in one embodiment, a nucleic acid probe that detects a target nucleic acid. At least one terminal of the probe-binding region of the target nucleic acid is a guanine base, and at least one cytosine base is present in the probe-binding region within 1 to 7 bases from the guanine base. The nucleic acid probe hybridizes with the probe-binding region of the target nucleic acid. The nucleic acid probe is provided with an oligonucleotide having at a terminal thereof a cytosine base opposite from the guanine base and is also provided with a fluorescent dye bonded to the cytosine base. The fluorescent dye is a fluorescent dye that is quenched by interaction with guanine base. The oligonucleotide is completely complementary with the nucleic acid of the probe-binding region excluding the cytosine base present within 1 to 7 bases from the terminal guanine base, and the base in the oligonucleotide opposite from the cytosine base that, of the cytosine bases, is nearest to the terminal guanine base, is a base lacking a fluorescence quenching activity.
(FR) La présente invention concerne, dans un mode de réalisation, une sonde d'acide nucléique qui détecte un acide nucléique cible. Au moins une terminaison de la région de fixation de la sonde de l'acide nucléique cible est une base guanine, et au moins une base cytosine est présente dans la région de fixation de la sonde dans 1 à 7 bases de la base guanine. La sonde d'acide nucléique s'hybride avec la région de fixation de la sonde de l'acide nucléique cible. La sonde d'acide nucléique est dotée d'un oligonucléotide présentant, à une de ses terminaisons, une base cytosine opposée à la base guanine, et est également dotée d'un colorant fluorescent lié à la base cytosine. Le colorant fluorescent est un colorant fluorescent qui subit une extinction par interaction avec la base guanine. L'oligonucléotide est totalement complémentaire à l'acide nucléique de la région de fixation de la sonde excluant la base cytosine présente dans 1 à 7 bases issues de la base guanine de terminaison, et la base dans l'oligonucléotide opposée à la base cytosine qui, parmi les bases cytosine, est plus proche de la base guanine de terminaison, est une base dépourvue d'une activité d'extinction de fluorescence.
(JA) 本発明は、一実施形態において、標的核酸を検出する核酸プローブである。標的核酸のプローブ結合領域の少なくとも一方の末端がグアニン塩基であり、かつ、該グアニン塩基から1~7塩基以内の前記プローブ結合領域にシトシン塩基が1つ以上存在する。核酸プローブは標的核酸のプローブ結合領域にハイブリダイズする。核酸プローブは、該グアニン塩基に対向するシトシン塩基を末端に有するオリゴヌクレオチドと、該シトシン塩基に結合された蛍光色素と、を備える。該蛍光色素は、グアニン塩基との相互作用により消光する蛍光色素である。前記オリゴヌクレオチドは、前記末端グアニン塩基から1~7塩基以内に存在するシトシン塩基を除いて前記プローブ結合領域の核酸と完全に相補的であり、該シトシン塩基のうち前記末端グアニン塩基に最も近接するシトシン塩基に対向する前記オリゴヌクレオチド上の塩基は蛍光の消光作用を持たない塩基である。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
CA3012815CN108603222EP3415636US20190055600