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1. (WO2017135396) PROBE SET FOR HLA GENOTYPING BY CAPTURE METHOD WITHOUT USING PCR, AND TYPING METHOD IN WHICH SAME IS USED
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/135396 International Application No.: PCT/JP2017/003902
Publication Date: 10.08.2017 International Filing Date: 03.02.2017
IPC:
C12N 15/09 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
ジェノダイブファーマ株式会社 GENODIVE PHARMA INC. [JP/JP]; 神奈川県横浜市泉区緑園1-19-35 1-19-35 Ryokuen, Izumi-ku, Yokohama-shi, Kanagawa 2450002, JP
Inventors:
細道 一善 HOSOMICHI, Kazuyoshi; JP
猪子 英俊 INOKO, Hidetoshi; JP
田嶋 敦 TAJIMA, Atsushi; JP
井ノ上 逸朗 Inoue, Itsuro; JP
Agent:
内田直人 UCHIDA, Naoto; JP
Priority Data:
2016-01894203.02.2016JP
Title (EN) PROBE SET FOR HLA GENOTYPING BY CAPTURE METHOD WITHOUT USING PCR, AND TYPING METHOD IN WHICH SAME IS USED
(FR) JEU DE SONDES POUR GÉNOTYPAGE HLA PAR PROCÉDÉ DE CAPTURE SANS UTILISATION DE PCR, ET PROCÉDÉ DE TYPAGE DANS LEQUEL IL EST UTILISÉ
(JA) PCRを用いないキャプチャー法によるHLA遺伝子タイピング用プローブセット及びそれを用いたタイピング方法
Abstract:
(EN) In the present invention, a novel probe set specialized to the HLA region has been designed, the probe set overcoming errors of HLA typing in PCR and the drawbacks of conventional sequence capture methods. Using the probe set makes it possible to process multiple specimens simultaneously as well as to comprehensively capture genes of the HLA region. Additionally including a probe for HLA pseudogenes in the probe set enables precise typing in which it is possible to specifically and efficiently capture each HLA gene. The present invention pertains to: a probe set for HLA genotyping including pseudogenes, the probe set comprising oligonucleotides each having a base sequence shown in SEQ ID NOS: 1-355; and a method for HLA genotyping including pseudogenes in which the probe set is used.
(FR) Dans la présente invention, un nouveau jeu de sondes spécialisé pour la région HLA a été conçu, le jeu de sondes surmontant les erreurs de typage HLA dans la PCR et les inconvénients des procédés classiques de capture de séquence. L’utilisation du jeu de sondes rend possible le traitement simultané d’échantillons multiples et la capture complète de gènes de la région HLA. De plus, l’introduction d’une sonde pour les pseudogènes HLA dans le jeu de sondes permet un typage précis, au moyen duquel il est possible de capturer spécifiquement et efficacement chaque gène HLA. La présente invention concerne : un jeu de sondes pour génotypage HLA comprenant des pseudogènes, le jeu de sondes comprenant des oligonucléotides ayant chacun une séquence de base représentée dans SEQ ID nos : 1 à 355 ; et un procédé de génotypage HLA comprenant des pseudogènes dans lesquels le jeu de sondes est utilisé.
(JA) 本不明は、PCR法におけるHLAタイピングのエラー並びに従来のシークエンスキャプチャー法の欠点を克服した、HLA領域に特化された新規なプローブセットをデザインした。当該プローブセットを用いることによって、多検体を同時に処理できるとともに、HLA領域の遺伝子を網羅的に捕捉できる。当該プローブセットにHLA偽遺伝子に対するプローブも含めることにより、各HLA遺伝子の捕捉をそれぞれ特異的、かつ効率よく行える精密なタイピングが可能になる。本発明は、配列番号:1~355に示す塩基配列を各々有するオリゴヌクレオチドからなる、偽遺伝子を含むHLA遺伝子タイピング用プローブセット及び当該プローブセットを用いた、偽遺伝子を含むHLA遺伝子タイピング方法に関する。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)