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1. (WO2017135309) MICRO- AND NANOCONTACT PRINTING WITH AMINOSILANES: PATTERNING SURFACES OF MICROFLUIDIC DEVICES FOR MULTI- PLEXED BIOASSAYS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/135309 International Application No.: PCT/JP2017/003621
Publication Date: 10.08.2017 International Filing Date: 01.02.2017
IPC:
G01N 33/547 (2006.01) ,C12M 1/00 (2006.01) ,G01N 33/543 (2006.01) ,G01N 37/00 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
544
the carrier being organic
545
Synthetic resin
547
with antigen or antibody attached to the carrier via a bridging agent
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
37
Details not covered by any other group of this subclass
Applicants:
OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL CORPORATION [JP/JP]; 1919-1, Tancha, Onna-son, Kunigami-gun, Okinawa 9040495, JP
Inventors:
FRIED, Amy Shen; JP
RICOULT, Sebastien Georg Gabriel; JP
Agent:
TSUKUNI & ASSOCIATES; KOJIMACHI BUSINESS CENTER 5-3-1, Kojimachi, Chiyoda-ku, Tokyo 1020083, JP
YAMAMURA, Daisuke; JP
MORITA, Yoshiko; JP
SHIOMI, Atsushi; JP
Priority Data:
62/290,06702.02.2016US
Title (EN) MICRO- AND NANOCONTACT PRINTING WITH AMINOSILANES: PATTERNING SURFACES OF MICROFLUIDIC DEVICES FOR MULTI- PLEXED BIOASSAYS
(FR) IMPRESSION PAR MICROCONTACT ET NANOCONTACT AVEC DES AMINOSILANES : FORMATION DE MOTIFS SUR DES SURFACES DE DISPOSITIFS MICROFLUIDIQUES POUR ESSAIS BIOLOGIQUES MULTIPLEXÉS
Abstract:
(EN) It is an object of the present invention to achieve rapid surface patterning of biomolecules within microfluidic devices with high reproducibility. In this work, we present a new means of creating micro- and nano-patterns of aminosilanes within microfluidic devices via an aqueous based microcontact printing technique. To minimize the diffusion of molecules into the PDMS stamp, we use water as the inking solvent and enforce short incubation and contact times during the printing process to preserve the pre-defined resolution of patterned features. These patterns then serve as the building block to couple multiple biomolecules in solution onto a single surface for subsequent bioassays.
(FR) Un objectif de la présente invention est d'obtenir une formation de motifs de surface rapide de biomolécules dans des dispositifs microfluidiques à haute reproductibilité. Dans cette invention, un nouveau moyen de création de micro-motifs et nano-motifs d'aminosilanes dans des dispositifs microfluidiques par une technique d'impression par microcontact à base aqueuse est présenté. Afin de minimiser la diffusion de molécules dans l'indicateur PDMS, de l'eau est utilisée comme solvant d'encrage et des temps d'incubation et de contact courts sont appliqués pendant le processus d'impression pour préserver la résolution prédéfinie d'éléments à motifs. Les motifs servent ensuite de bloc de construction pour coupler de multiples biomolécules en solution sur une surface unique pour des essais biologiques ultérieurs.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
US20190030530