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1. (WO2017132909) METHOD FOR SEPARATING TARGET CELL FROM BLOOD SAMPLE AND USE THEREOF
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/132909 International Application No.: PCT/CN2016/073375
Publication Date: 10.08.2017 International Filing Date: 03.02.2016
IPC:
C12N 5/078 (2010.01) ,C12N 5/073 (2010.01) ,C12N 15/10 (2006.01) ,C12M 1/42 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 33/53 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
078
Cells from blood or from the immune system
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
073
Embryonic cells or tissues; Foetal cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
42
Apparatus for the treatment of micro-organisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
Applicants:
深圳华大生命科学研究院 BGI SHENZHEN [CN/CN]; 中国广东省深圳市 盐田区北山工业区综合楼 Main Building, Beishan Industrial Zone, Yantian District Shenzhen, Guangdong 518083, CN
Inventors:
朱珠 ZHU, Zhu; CN
刘萍 LIU, Ping; CN
顾颖 GU, Ying; CN
康雄斌 KANG, Xiongbin; CN
夏军 XIA, Jun; CN
邱咏 QIU, Yong; CN
Agent:
北京清亦华知识产权代理事务所(普通合伙) TSINGYIHUA INTELLECTUAL PROPERTY LLC; 中国北京市 海淀区清华园清华大学照澜院商业楼301室 Room 301, Trade Building, Zhaolanyuan Tsinghua University, Qinghuayuan Haidian District, Beijing 100084, CN
Priority Data:
Title (EN) METHOD FOR SEPARATING TARGET CELL FROM BLOOD SAMPLE AND USE THEREOF
(FR) PROCÉDÉ POUR SÉPARER UNE CELLULE CIBLE À PARTIR D'UN ÉCHANTILLON DE SANG ET UTILISATION CORRESPONDANTE
(ZH) 从血液样本中分离靶细胞的方法及其用途
Abstract:
(EN) The present invention provides a method for obtaining a target cell from a blood sample and a use thereof. The method comprises: (1) subjecting the blood sample to immunomagnetic bead sorting to obtain a first sorted product comprising the target cell; (2) subjecting the first sorted product to flow cytometric sorting to obtain a second sorted product comprising the target cell; and (3) separating the second sorted product by a mouth pipetting method to obtain the target cell in a unicellular form.
(FR) La présente invention concerne un procédé pour obtenir une cellule cible à partir d'un échantillon de sang et une utilisation correspondante. Le procédé consiste à : (1) soumettre l'échantillon de sang à un tri par billes immunomagnétiques pour obtenir un premier produit trié comprenant la cellule cible ; (2) soumettre le premier produit trié à un tri cytométrique en flux pour obtenir un deuxième produit trié comprenant la cellule cible ; et (3) séparer le deuxième produit trié par un procédé de pipetage à la bouche pour obtenir la cellule cible sous une forme unicellulaire.
(ZH) 提供了从血液样本中获得靶细胞的方法及其用途。该方法包括:(1)将血液样本进行免疫磁珠分选,以便获得含有该靶细胞的第一分选产物;(2)将该第一分选产物进行流式细胞分选,以便获得含有该靶细胞的第二分选产物;以及(3)采用口吸管法分离该第二分选产物,以便获得呈单细胞形式的该靶细胞。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)
Also published as:
CN108473955EP3412769