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1. (WO2017132376) HYBRID PROMOTER AND USES THEREOF
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/132376 International Application No.: PCT/US2017/015130
Publication Date: 03.08.2017 International Filing Date: 26.01.2017
IPC:
C12N 15/86 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
86
Viral vectors
Applicants:
JUST BIOTHERAPEUTICS, INC. [US/US]; 401 Terry Avenue North Seattle, Washington 98109, US
Inventors:
MCGREW, Jeffrey T.; US
SMIDT, Pauline S.; US
ONG, E-Ching; US
Agent:
STEINBERG, Nisan A.; US
Priority Data:
62/388,39127.01.2016US
Title (EN) HYBRID PROMOTER AND USES THEREOF
(FR) PROMOTEUR HYBRIDE ET SES UTILISATIONS
Abstract:
(EN) A hybrid promoter for recombinant expression of proteins of interest is disclosed that combines a mCMV enhancer sequence with a rat EF-1alpha intron sequence. Also disclosed are an expression cassette containing the hybrid promoter and a recombinant expression vector containing the expression cassette. A mammalian host cell, which comprises the recombinant expression vector is also disclosed, as is a method of producing a protein of interest that employs the mammalian host cell, optionally involving tetracycline-inducible expression of the protein.
(FR) La présente invention concerne un promoteur hybride destiné à l'expression recombinée de protéines d'intérêt qui combine une séquence activatrice de mCMV avec une séquence d'intron d'EF-1alpha de rat. L'invention concerne également une cassette d'expression contenant le promoteur hybride et un vecteur d'expression recombiné contenant la cassette d'expression. L'invention concerne également une cellule hôte de mammifère, qui comprend le vecteur d'expression recombiné, ainsi qu'un procédé de production d'une protéine d'intérêt qui utilise la cellule hôte de mammifère, comprenant éventuellement l'expression inductible par tétracycline de ladite protéine.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP3408397US20190055580