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1. WO2017132266 - MICROFLUIDIC PACKAGE AND METHOD OF MAKING THE SAME

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[ EN ]

CLAIMS

1. An encapsulated microfluidic package comprising:

a device comprising an active area and an inactive area, wherein the active area comprises one or more capture probes;

a cradle configured to support the device;

a first bonding surface disposed on a portion of a surface of the cradle, wherein the first bonding surface comprises a first reactive group;

a lid comprising a recess, an upper surface, and a second bonding surface disposed on a lower surface of the lid, wherein the recess is configured to be disposed above the active area and wherein the second bonding surface comprises a second reactive group configured to react with the first reactive group; and

a first fluidic seal between the first and second bonding surfaces, wherein the first fluidic seal results from a reaction between the first and second reactive groups and wherein the first fluidic seal is formed in the presence of the one or more capture probes.

2. The package of claim 1 , wherein the second bonding surface is disposed above the first bonding surface and disposed above a portion of a surface of the inactive area.

3. The package of claim 2, further comprising a third bonding surface disposed on the portion of the surface of the inactive area, wherein the third bonding surface comprises a third reactive group configured to react with the second reactive group.

4. The package of claim 3, wherein the fluidic seal results from a reaction between the first and second reactive groups and a reaction between the second and third reactive groups.

5. The package of claim 1 , wherein the lid comprises a first polymer, the cradle comprises a second polymer, and the first and second polymers are same or different.

6. The package of claim 1 , wherein the lid and/or the cradle comprises a functionalized polymer comprising polynorbomene, polycarbonate, or copolymers thereof.

7. The package of claim 1, wherein the second reactive group comprises an amino group and/or a thio group.

8. The package of claim 7, wherein the first reactive group comprises an amido group and/or an epoxide group.

9. The package of claim 1, wherein the first fluidic seal comprises a covalent bond between the first and second reactive groups.

10. The package of claim 1, wherein the one or more capture probes comprises an antibody, an aptamer, a nucleic acid, a protein, a receptor, and/or an enzyme, or fragments thereof.

11. The package of claim 1 , wherein the lid comprises a cover and a plurality of pillars, and wherein the second bonding surface is disposed on a surface of at least one of the plurality of pillars.

12. The package of claim 1, further comprising a protected surface disposed on a portion of the surface of the inactive area, wherein the protected surface comprises a protecting group configured to reduce binding of a chemical or biochemical moiety to the protected surface.

13. The package of claim 1, further comprising:

an intermediate layer comprising a further binding surface, wherein the intermediate layer is configured to be disposed above the upper surface of the lid, wherein the upper surface of the lid comprises the second reactive group, and wherein the further binding surface comprises a further reactive group configured to react with the second reactive group of the upper surface; and

a second fluidic seal between the intermediate layer and the upper surface of the lid.

14. The package of claim 13, wherein the intermediate layer further comprises one or more inlets, vias, or chambers configured to provide fluidic communication with the recess.

15. An encapsulated microfluidic package comprising:

a device comprising an active area and an inactive area, wherein the active area comprises one or more capture probes;

a first bonding surface disposed on a portion of a surface of the inactive area, wherein the first bonding surface comprises a first reactive group;

a lid comprising a recess and a second bonding surface, wherein the recess is configured to be disposed above the active area and wherein the second bonding surface comprises a second reactive group configured to react with the first reactive group; and

a first fluidic seal between the first and second bonding surfaces, wherein the seal results from a reaction between the first and second reactive groups and wherein the seal is formed in the presence of the one or more capture probes.

16. The package of claim 15, wherein the lid comprises a cover and a plurality of pillars, and wherein the second bonding surface is disposed on a surface of at least one of the plurality of pillars.

17. A method of making an encapsulated microfluidic package, the method comprising:

functionalizing a portion of a device to provide a first bonding surface comprising a first reactive group, wherein the device comprises an active area and an inactive area, and wherein the active area comprises one or more capture probes; functionalizing a lid to provide a second bonding surface comprising a second reactive group, wherein the lid comprises a recess, an upper surface, and the second bonding surface disposed on a lower surface of the lid, wherein the recess is configured to be disposed above the active area, and wherein the second reactive group is configured to react with the first reactive group; and

attaching the second bonding surface of the lid to the first bonding surface of the device, thereby forming a first fluidic seal, wherein the first fluidic seal results from a reaction between the first and second reactive groups and wherein the first fluidic seal is formed in the presence of the one or more capture probes.

18. The method of claim 17, further comprising, prior to the attaching step:

functionalizing the inactive area of the device to provide a protected surface comprising a protecting group.

19. A method of making an encapsulated microfiuidic package, the method comprising:

attaching a device to a cradle, wherein the device comprises an active area and an inactive area, and wherein the active area comprises one or more capture probes; functionalizing a portion of the cradle to provide a first bonding surface comprising a first reactive group;

functionalizing a lid to provide a second bonding surface comprising a second reactive group, wherein the lid comprises a recess, an upper surface, and the second bonding surface disposed on a lower surface of the lid, wherein the recess is configured to be disposed above the active area, and wherein the second reactive group is configured to react with the first reactive group; and

attaching the second bonding surface of the lid to the first bonding surface of the cradle, thereby forming a first fluidic seal, wherein the first fluidic seal results from a reaction between the first and second reactive groups and wherein the first fluidic seal is formed in the presence of the one or more capture probes.

20. The method of claim 19, further comprising:

functionalizing a portion of a device to provide a third bonding surface comprising a third reactive group, wherein the third reactive group is configured to react with the second reactive group; and/or

functionalizing a portion of the inactive area of the device to provide a protected surface comprising a protecting group.

21. A method of making an encapsulated microfiuidic package, the method comprising:

forming at least two pillars on an inactive area of a device, wherein the at least two pillars surround an active area of the device;

functionalizing a portion of each of the at least two pillars to provide a first bonding surface including a first reactive group;

functionalizing a cover and/or a lid to provide a second bonding surface comprising a second reactive group, wherein the second reactive group is configured to react with the first reactive group; and

attaching the second bonding surface of the cover to the first bonding surface of the pillar, thereby providing a lid having a recess disposed above the active area and forming a first fluidic seal, wherein the first fluidic seal results from a reaction between the first and second reactive groups.