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1. (WO2017131022) METHOD FOR DETECTING GLYCOPROTEIN
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/131022 International Application No.: PCT/JP2017/002517
Publication Date: 03.08.2017 International Filing Date: 25.01.2017
IPC:
C12Q 1/37 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/53 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
37
involving peptidase or proteinase
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
Applicants:
株式会社J-オイルミルズ J-OIL MILLS, INC. [JP/JP]; 東京都中央区明石町8番1号 8-1, Akashi-cho, Chuo-ku Tokyo 1040044, JP
Inventors:
小林 夕香 KOBAYASHI Yuka; JP
西藤 桂子 SAITO Keiko; JP
上野 泰 UENO Yasushi; JP
Agent:
中嶋 伸介 NAKAJIMA Shinsuke; JP
Priority Data:
2016-01318827.01.2016JP
Title (EN) METHOD FOR DETECTING GLYCOPROTEIN
(FR) PROCÉDÉ DE DÉTECTION D'UNE GLYCOPROTÉINE
(JA) 糖タンパク質の検出方法
Abstract:
(EN) [Problem] To provide a method that increases the detection level of a reactant generated through a reaction between a glycoprotein and a carbohydrate-binding compound in order to detect the glycoprotein with high precision. [Solution] A method for detecting a glycoprotein according to the present invention comprises: a step in which a sample containing a glycoprotein is treated with a protease; a step in which a carbohydrate-binding compound having affinity with carbohydrate chains comprising the glycoprotein that has been treated with the protease is made to act on the glycoprotein, thereby generating a reactant of the glycoprotein and the carbohydrate-binding compound; and a step in which the reactant is detected. The carbohydrate-binding compound is preferably a carbohydrate-binding protein.
(FR) La présente invention vise à fournir un procédé qui augmente le niveau de détection d'un réactif généré par une réaction entre une glycoprotéine et un composé se liant aux glucides pour détecter la glycoprotéine avec une précision élevée. L'invention concerne par conséquent un procédé de détection d'une glycoprotéine selon la présente invention, qui comprend : une étape dans laquelle un échantillon contenant une glycoprotéine est traité avec une protéase ; une étape dans laquelle on fait agir, sur la glycoprotéine, un composé se liant aux glucides présentant une affinité pour les chaînes glucidiques comprenant la glycoprotéine traitée avec la protéase, ce qui permet ainsi de générer un réactif de la glycoprotéine et du composé à liaison glucidique ; et une étape dans laquelle le réactif est détecté. Le composé se liant aux glucides est de préférence une protéine se liant aux glucides.
(JA) 【課題】糖タンパク質を高精度に検出するために、糖タンパク質と糖結合化合物との反応による反応物の検出レベルを増大させる方法を提供する。 【解決手段】本発明の糖タンパク質の検出方法は、該糖タンパク質を含む試料をプロテアーゼ処理する工程、前記プロテアーゼ処理された糖タンパク質に、前記糖タンパク質の有する糖鎖と親和性を有する糖結合化合物を作用させて、該糖タンパク質と該糖結合化合物との反応物を得る工程、及び、前記反応物を検出する工程を含む。糖結合化合物は、好ましくは糖結合タンパク質である。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)