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1. (WO2017130829) MICROARRAY, METHOD FOR MANUFACTURING MICROARRAY, INSPECTION METHOD, AND INSPECTION KIT
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/130829 International Application No.: PCT/JP2017/001724
Publication Date: 03.08.2017 International Filing Date: 19.01.2017
IPC:
G01N 33/53 (2006.01) ,C12M 1/34 (2006.01) ,C12Q 1/02 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/80 (2006.01) ,G01N 37/00 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
80
involving blood groups or blood types
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
37
Details not covered by any other group of this subclass
Applicants:
学校法人北里研究所 SCHOOL JURIDICAL PERSON THE KITASATO INSTITUTE [JP/JP]; 東京都港区白金5丁目9番1号 9-1, Shirokane 5-chome, Minato-ku, Tokyo 1088641, JP
Inventors:
鉢村 和男 HACHIMURA Kazuo; JP
長塩 亮 NAGASHIO Ryo; JP
佐藤 雄一 SATO Yuichi; JP
大谷 慎一 OTANI Shinichi; JP
狩野 有作 KANOH Yuhsaku; JP
Agent:
棚井 澄雄 TANAI Sumio; JP
飯田 雅人 IIDA Masato; JP
Priority Data:
2016-01176225.01.2016JP
Title (EN) MICROARRAY, METHOD FOR MANUFACTURING MICROARRAY, INSPECTION METHOD, AND INSPECTION KIT
(FR) MICROPUCE, PROCÉDÉ DE FABRICATION D'UNE MICROPUCE, PROCÉDÉ D'INSPECTION ET KIT D'INSPECTION
(JA) マイクロアレイ、マイクロアレイの製造方法、検査方法、及び検査キット
Abstract:
(EN) The present invention provides a microarray with which it is possible to simultaneously react a blood sample and a plurality of types of reagent red blood cells in the same reaction phase, and that enables detection and identification of irregular antibodies in a simple manner and at a high sensitivity. This microarray for detecting and identifying irregular antibodies in a blood sample is characterized in that sets of red blood cells or red blood cell ghosts presenting a known antigen on the cell surface are immobilized in a regular arrangement on a substrate. This inspection method for inspecting irregular antibodies in a blood sample is provided with: a step for bringing a blood sample collected from a subject into contact with the microarray and performing a first antigen-antibody interaction; a step for cleaning the microarray, bringing a labeled anti-human antibody into contact therewith, and performing a second antigen-antibody interaction; and a step for detecting the labeled anti-human antibody.
(FR) La présente invention concerne une micropuce avec laquelle il est possible de simultanément mettre en réaction un échantillon de sang et une pluralité de globules rouges réactifs dans la même phase de réaction, et qui permet de détecter et d'identifier des anticorps irréguliers de manière simple et avec une sensibilité élevée. Cette micropuce de détection et d'identification d'anticorps irréguliers dans un échantillon de sang est caractérisée en ce que des ensembles de globules rouges ou de fantômes de globules rouges présentant un antigène connu sur la surface cellulaire sont immobilisés selon un agencement régulier sur un substrat. Ce procédé d'inspection pour inspecter des anticorps irréguliers dans un échantillon de sang comprend : une étape de mise en contact d'un échantillon de sang prélevé sur un sujet avec la micropuce et de réalisation d'une première interaction antigène-anticorps ; une étape de nettoyage de la micropuce, en mettant en contact un anticorps anti-humain marqué avec celle-ci, et en réalisant une seconde interaction antigène-anticorps; et une étape de détection de l'anticorps anti-humain marqué.
(JA) 本発明は、血液試料と複数種類の試薬赤血球とを同一反応相にて同時に反応させることができ、簡便且つ高感度に不規則性抗体の検出及び同定を可能とするマイクロアレイを提供する。本発明のマイクロアレイは、血液試料中の不規則性抗体を検出及び同定するためのマイクロアレイであって、基板上に細胞表面上に既知の抗原を提示する赤血球又は赤血球ゴーストのセットが規則的に固定化されたことを特徴とする。本発明の検査方法は、血液試料中の不規則性抗体を検査するための方法であって、前記マイクロアレイに、被験者から採取した血液試料を接触させ、第1の抗原抗体反応を行う工程と、前記マイクロアレイを洗浄し、標識された抗ヒト抗体を接触させ、第2の抗原抗体反応を行う工程と、前記標識された抗ヒト抗体を検出する工程と、を備える。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)