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1. WO2017130610 - FUSION PROTEIN AND METHOD FOR DETECTING ANTIGEN USING SAME

Publication Number WO/2017/130610
Publication Date 03.08.2017
International Application No. PCT/JP2016/088061
International Filing Date 21.12.2016
IPC
C07K 19/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
19Hybrid peptides
C07K 16/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C12N 9/24 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
24acting on glycosyl compounds (3.2)
C12N 15/09 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12Q 1/34 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
34involving hydrolase
CPC
C07K 16/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
C07K 19/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
19Hybrid peptides
C12N 15/09
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12N 9/24
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
24acting on glycosyl compounds (3.2)
C12Q 1/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
Applicants
  • 国立大学法人東京工業大学 TOKYO INSTITUTE OF TECHNOLOGY [JP]/[JP]
Inventors
  • 上田 宏 UEDA, Hiroshi
  • 董 金華 DONG, Jinhua
Agents
  • 野村 健一 NOMURA, Kenichi
  • 間山 世津子 MAYAMA, Setsuko
Priority Data
2016-01147525.01.2016JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) FUSION PROTEIN AND METHOD FOR DETECTING ANTIGEN USING SAME
(FR) PROTÉINE DE FUSION ET PROCÉDÉ DE DÉTECTION D'ANTIGÈNE UTILISANT LADITE PROTÉINE
(JA) 融合タンパク質及びそれを用いた抗原の検出方法
Abstract
(EN)
In order to establish an antigen detection means capable of simply and quickly detecting an antigen and having excellent detection sensitivity, etc., this fusion protein contains an enzyme variant which is activated by multimeric formation, a linker peptide linked to the enzyme variant, and the VH domain or VL domain of an antibody which binds to the linker peptide, and is characterized in that the enzyme variant has an introduced mutation which reduces linking affinity between monomers.
(FR)
L’invention vise à établir un moyen de détection d'antigène apte à détecter simplement et rapidement un antigène et présentant une excellente sensibilité de détection, etc. La protéine de fusion comprend un variant enzymatique qui est activé par la formation de multimères, un peptide lieur lié au variant enzymatique, et le domaine VH ou le domaine VL d'un anticorps qui se relie au peptide lieur, et est caractérisée en ce que le variant enzymatique présente une mutation introduite qui réduit l'affinité de liaison entre les monomères.
(JA)
簡便かつ迅速に抗原を検出でき、また、検出感度などの面においても優れた抗原の検出手段の確立を目的として、多量体の形成により活性化する酵素の変異体、この酵素の変異体と結合するリンカーペプチド、及びこのリンカーペプチドと結合する抗体のVH領域又はVL領域を含む融合タンパク質であって、前記酵素の変異体が、単量体間の結合親和性を低下させる変異が導入された変異体であることを特徴とする融合タンパク質を提供する。
Also published as
Latest bibliographic data on file with the International Bureau