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1. (WO2017129739) REDUCING CADMIUM ACCUMULATION IN FIELD GROWN TOBACCO PLANTS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/129739 International Application No.: PCT/EP2017/051761
Publication Date: 03.08.2017 International Filing Date: 27.01.2017
IPC:
A01H 5/12 (2006.01) ,C07K 14/415 (2006.01) ,C12N 15/82 (2006.01) ,A24B 15/10 (2006.01)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
12
Leaves
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415
from plants
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
A HUMAN NECESSITIES
24
TOBACCO; CIGARS; CIGARETTES; SMOKERS' REQUISITES
B
MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
15
Chemical features or treatment of tobacco; Tobacco substitutes
10
Chemical features of tobacco products or tobacco substitutes
Applicants:
PHILIP MORRIS PRODUCTS S.A. [CH/CH]; Quai Jeanrenaud 3 2000 Neuchatel, CH
Inventors:
BOVET, Lucien; CH
LIEDSCHULTE, Verena; CH
Agent:
LLOYD, John Scott; GB
Priority Data:
16153529.929.01.2016EP
Title (EN) REDUCING CADMIUM ACCUMULATION IN FIELD GROWN TOBACCO PLANTS
(FR) RÉDUCTION D'ACCUMULATION DE CADMIUM DANS DES PLANTES DE TABAC CULTIVÉES DANS UN CHAMP
Abstract:
(EN) There is described herein a mutant plant or part thereof having at least partially reduced expression or activity of at least two heavy metal ATPases (HMAs), said two HMAs comprising, consisting or consisting essentially of : (i) polypeptides having at least 65% sequence identity to SEQ ID NO:1 and SEQ ID NO:2; (ii) polynucleotides encoding the polypeptides set forth in (i); or (iii) polynucleotides having at least 65% sequence identity to SEQ ID NO:3 and SEQ ID NO:4 encoding HMAs; wherein the expression or activity of one of the HMAs set forth in (i) or (ii) or (iii) is partially reduced or lost and the expression or activity of one of the HMAs set forth in (i) or (ii) or (iii) is lost as compared to a control plant; and wherein the mutant plant or part thereof exhibits at least a 27% reduction, as compared to the control plant, in the accumulation of cadmium in leaf when the mutant plant is grown in the field in the presence of naturally or non-naturally occurring cadmium.
(FR) L'invention concerne une plante mutante ou une partie de cette dernière ayant une expression ou activité au moins partiellement réduite d'au moins deux ATPases de métaux lourds (HMA), lesdits deux HMA comprenant, comportant ou comportant essentiellement : (i) des polypeptides ayant au moins 65 % d'identité de séquence avec SEQ ID NO : 1 et SEQ ID NO : 2 ; (ii) des polynucléotides codant les polypeptides présentés dans (i) ; ou (iii) des polynucléotides ayant au moins 65 % d'identité de séquence avec SEQ ID NO : 3 et SEQ ID NO : 4 codant HMA ; l'expression ou activité de l'un des HMA présentés dans (i), (ii) ou (iii) étant partiellement réduite ou perdue et l'expression ou activité de l'un des HMA présentés dans (i) ou (ii) ou (iii) étant perdue par comparaison avec une plante témoin ; et la plante mutante ou une partie de cette dernière présentant une réduction d'au moins 27 %, par comparaison avec la plante témoin, de l'accumulation de cadmium dans une feuille lorsque la plante mutante est cultivée dans le champ en présence de cadmium naturel ou non-naturel.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AR107478AU2017211970CA3010142KR1020180107123CN108697059EP3407704
ID2018/12759BR112018014765EA201891707PH1/2018/501304