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1. (WO2017129136) D-LACTATE DEHYDROGENASE, ENGINEERED STRAIN CONTAINING SAME AND CONSTRUCTION AND USE THEREOF
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/129136 International Application No.: PCT/CN2017/072647
Publication Date: 03.08.2017 International Filing Date: 25.01.2017
IPC:
C12N 9/04 (2006.01) ,C12N 15/53 (2006.01) ,C12N 1/21 (2006.01) ,C12P 7/56 (2006.01) ,C12R 1/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
04
acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
53
Oxidoreductases (1)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
21
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
40
containing a carboxyl group
56
Lactic acid
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
01
Bacteria or actinomycetales
07
Bacillus
10
Bacillus licheniformis
Applicants:
上海交通大学 SHANGHAI JIAO TONG UNIVERSITY [CN/CN]; 中国上海市 闵行区东川路800号 No.800 Dongchuan Road, Minhang District Shanghai 200240, CN
Inventors:
许平 XU, Ping; CN
李超 LI, Chao; CN
陶飞 TAO, Fei; CN
唐鸿志 TANG, Hongzhi; CN
Agent:
上海旭诚知识产权代理有限公司 SUNRAY INTELLECTUAL PROPERTY ATTORNEYS; 中国上海市 东方路710号汤臣金融大厦1212室郑立 ZHENG Li Suite 1212, Tomson Commercial Building 710 Dongfang Road Shanghai 200122, CN
Priority Data:
201610056883.727.01.2016CN
Title (EN) D-LACTATE DEHYDROGENASE, ENGINEERED STRAIN CONTAINING SAME AND CONSTRUCTION AND USE THEREOF
(FR) D-LACTATE DÉSHYDROGÉNASE, SOUCHE MODIFIÉE LA CONTENANT ET FABRICATION ET UTILISATION DE CETTE DERNIÈRE
(ZH) 一种D-乳酸脱氢酶、含有该酶的工程菌株及其构建和应用
Abstract:
(EN) Provided are a D-lactate dehydrogenase, an engineered strain containing same and the construction and use thereof. Also provided is a D-lactate dehydrogenase derived from Thermodesulfatator indicus, which has thermophily and thermostability. With the D-lactate dehydrogenase and the genetic engineering method, the fermentation product of the engineered Bacillus licheniformis is redirected from the naturally produced 2,3-butanediol to high-yielding optically pure D-lactic acid, and the optical purity of the produced D-lactic acid reaches 99.9%. The prices of the raw materials used in the fermentation are relatively low, and the fermentation state is between an anaerobic condition and a micro-aerobic condition.
(FR) L'invention concerne une D-lactate déshydrogénase, une souche modifiée la contenant et la fabrication et l'utilisation de cette dernière. L'invention concerne également une D-lactate déshydrogénase dérivée de Thermodesulfatator indicus, qui présente des qualités de thermophilie et de thermostabilité. Grâce à la D-lactate déshydrogénase et au procédé de génie génétique, le produit de fermentation du Bacillus licheniformis modifié est redirigé du 2,3-butanediol produit naturellement vers un acide D-lactique optiquement pur à un haut rendement, et la pureté optique de l'acide D-lactique atteint 99,9 %. Les prix des matières premières utilisées dans la fermentation sont relativement faibles, et l'état de fermentation se situe entre un état anaérobie et un état micro-aérobie.
(ZH) 提供了一种D-乳酸脱氢酶、含有该酶的工程菌株及其构建和应用。还提供了一个来自热脱硫杆菌属(Thermodesulfatator indicus)的D-乳酸脱氢酶,其具有嗜热性及热稳定性。利用该D-乳酸脱氢酶和所述的基因工程改造的方法,使得改造后的地衣芽孢杆菌的发酵产物从天然的产2,3-丁二醇重定向为高产光学纯D-乳酸,所产的D-乳酸光学纯度达99.9%;发酵所用的原料价格较低,发酵状态介于厌氧和微氧之间。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)
Also published as:
US20190040425