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1. (WO2017124100) METHODS FOR EXTENDING THE REPLICATIVE CAPACITY OF SOMATIC CELLS DURING AN EX VIVO CULTIVATION PROCESS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/124100 International Application No.: PCT/US2017/013782
Publication Date: 20.07.2017 International Filing Date: 17.01.2017
IPC:
C12N 15/85 (2006.01) ,C12N 5/077 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
Applicants:
MEMPHIS MEATS, INC. [US/US]; 1933 Davis Street Suite 312 San Leandro, California 94577, US
Inventors:
GENOVESE, Nicholas; US
DESMET, Danielle Nicole; US
SCHULZE, Eric; US
Agent:
MADHURI, Roy; US
Priority Data:
62/278,86914.01.2016US
62/361,86713.07.2016US
Title (EN) METHODS FOR EXTENDING THE REPLICATIVE CAPACITY OF SOMATIC CELLS DURING AN EX VIVO CULTIVATION PROCESS
(FR) PROCÉDÉS D'EXTENSION DE CAPACITÉ DE RÉPLICATION DE CELLULES SOMATIQUES PENDANT UN PROCESSUS DE CULTURE EX VIVO
Abstract:
(EN) A product and process for extending the replicative capacity of metazoan somatic cells using targeted genetic amendments to abrogate inhibition of cell-cycle progression during replicative senescence and derive clonal cell lines for scalable applications and industrial production of metazoan cell biomass. An insertion or deletion mutation using guide RNAs targeting the first exon of the transcript encoding each protein is created using CRISPR/Cas9. Targeted amendments result in inactivation of p15 and p16 proteins which increases the proliferative capacity of the modified cell populations relative to their unaltered parental populations. Combining these amendments with ancillary telomerase activity from a genetic construct directing expression of a telomerase protein homolog from a TERT gene, increases the replicative capacity of the modified cell populations indefinitely. One application is to manufacture skeletal muscle for dietary consumption using cells from the poultry species Gallus gallus; another is from the livestock species Bos taurus.
(FR) L'invention concerne un produit et un procédé pour l'extension de capacité de réplication de cellules somatiques métazoaires utilisant des modifications génétiques ciblées pour supprimer l'inhibition de progression de cycle cellulaire pendant une sénescence réplicative et obtenir des lignées cellulaires clonales pour des applications à échelle variable et la production industrielle de biomasse de cellule métazoaire. Une mutation par insertion ou délétion à l'aide d'ARN guides ciblant le premier exon du transcrit codant chaque protéine est créée à l'aide de CRISPR/Cas9. Des modifications ciblées entraînent l'inactivation de protéines p15 et p16, ce qui augmente la capacité de prolifération des populations cellulaires modifiées par rapport à leurs populations parentales non modifiées. La combinaison de ces modifications avec une activité de télomérase auxiliaire d'une construction génétique dirigeant l'expression d'un homologue de protéine télomérase d'un gène TERT augmente la capacité de réplication des populations cellulaires modifiées indéfiniment. Une application consiste à fabriquer un muscle squelettique pour une consommation alimentaire à l'aide de cellules de l'espèce de volaille Gallus gallus ; une autre étant l'espèce de bétail Bos taurus.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)