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1. (WO2017112471) METHODS, SYSTEMS, AND COMPOSITIONS FOR PROPAGATION OF A FERMENTATION MICROORGANISM
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We Claim:

1. A method for propagating a fermentation microorganism for fermentation comprising:

(a) providing a hydrolysate for fermentation, wherein the hydrolysate comprises at least 10 g/L ethanol and wherein the hydrolysate is generated according to a method comprising:

- ensiling a cellulosic biomass material for at least 24 hours to generate ethanol in the cellulosic biomass material to generate an ensiled cellulosic biomass material;

- pretreating the ensiled cellulosic biomass material with a solution comprising an alpha-hydroxy sulfonic acid to produce a pretreated material comprising ethanol; and

- introducing one or more cellulases to the pretreated material to produce the hydrolysate;

(b) providing a propagation reactor containing:

a propagation medium comprising: the hydrolysate in an amount of at least 25% by volume and glucose in an amount of greater than 14 g/L; and

a first cell mass of a fermentation microorganism; and

(c) propagating the first cell mass in the propagation medium for a time period to form a propagated culture comprising a second cell mass of the microorganism that is greater than the first cell mass.

2. The method of any preceding claim wherein at least 15 g/L of endogenous ethanol is produced by the fermentation microorganism during propagation.

3. The method of any preceding claim wherein the hydrolysate contains at least 20% total solids (TS).

4. The method of any preceding claim wherein the fermentation organism comprises a yeast.

5. The method of any preceding claim wherein the hydrolysate comprises at least 14 g/L exogenous ethanol.

6. The method of any preceding claim wherein the propagation medium comprises at least 50% hydrolysate by volume.

7. The method of any preceding claim further comprising:

providing the second cell mass of microorganism to the hydrolysate in a fermentation reactor; and

growing the microorganism in the fermentation reactor under anaerobic conditions to produce a fermentation product.

8. The method of any preceding claim further comprising a pre-propagation step, wherein the first cell mass provided to the propagation reactor has been grown in a pre-propagation reactor in a pre-propagation medium comprising hydrolysate in an amount of at least 25% by volume and glucose in an amount of greater than 14 g/L.

9. The method of any preceding claim wherein the pretreated material is substantially free of the a-hydroxy sulfonic acid.

10. The method of any preceding claim wherein the cellulosic biomass is selected from the group consisting of sorghum, sugar cane, corn, tropical corn, sugar beet, energy cane, and any combination thereof.