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1. (WO2017112471) METHODS, SYSTEMS, AND COMPOSITIONS FOR PROPAGATION OF A FERMENTATION MICROORGANISM
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/112471 International Application No.: PCT/US2016/066469
Publication Date: 29.06.2017 International Filing Date: 14.12.2016
IPC:
C12N 1/18 (2006.01) ,C12N 1/32 (2006.01) ,C12N 1/16 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
18
Baker's yeast; Brewer's yeast
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
32
Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
Applicants:
SHELL OIL COMPANY [US/US]; One Shell Plaza P.O. Box 2463 Houston, Texas 77252-2463, US (US)
SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ B.V. [NL/NL]; Carel van Bylandtlaan 30 NL-2596 The Hague, NL (AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BE, BF, BG, BH, BJ, BN, BR, BW, BY, BZ, CA, CF, CG, CH, CI, CL, CM, CN, CO, CR, CU, CY, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, FR, GA, GB, GD, GE, GH, GM, GN, GQ, GR, GT, GW, HN, HR, HU, ID, IE, IL, IN, IR, IS, IT, JP, KE, KG, KH, KM, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MC, MD, ME, MG, MK, ML, MN, MR, MT, MW, MX, MY, MZ, NA, NE, NG, NI, NL, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SI, SK, SL, SM, SN, ST, SV, SY, SZ, TD, TG, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, ZA, ZM, ZW)
Inventors:
PARKER, David Alexander; US
ZHANG, Kun; US
BOTELLA-FRANCO, Carolina; US
LIANG, Yi; US
ZUROFF, Trevor Roman; US
Agent:
LUONG, Huyen; US
Priority Data:
62/270,09721.12.2015US
Title (EN) METHODS, SYSTEMS, AND COMPOSITIONS FOR PROPAGATION OF A FERMENTATION MICROORGANISM
(FR) PROCÉDÉS, SYSTÈMES ET COMPOSITIONS POUR LA PROPAGATION D'UN MICROORGANISME DE FERMENTATION
Abstract:
(EN) Methods, systems, and compositions for propagating a fermentation microorganism that can have improved product yield during fermentation of a hydrolysate containing at least one growth inhibitor, including ethanol. In some embodiments, the method comprises (a) providing a generated according to a method comprising: - ensiling a cellulosic biomass material for at least 24 hours to generate ethanol in the cellulosic biomass material to generate an ensiled cellulosic biomass material; - pretreating the ensiled cellulosic biomass material with a solution comprising an alpha-hydroxysulfonic acid to produce a pretreated material comprising ethanol; and - introducing one or more cellulases to the pretreated material to produce the hydrolysate; (b) providing a propagation reactor containing: a propagation medium comprising: the hydrolysate in an amount of at least 25% by volume and glucose in an amount of greater than 14 g/L; and a first cell mass of a fermentation microorganism.
(FR) L'invention concerne des procédés, des systèmes et des compositions pour la propagation d'un microorganisme de fermentation qui peut présenter un rendement en produit amélioré pendant la fermentation d'un hydrolysat contenant au moins un inhibiteur de croissance, notamment de l'éthanol. Dans certains modes de réalisation, le procédé consiste : (a) à fournir un produit selon un procédé comprenant l'ensilage d'une biomasse cellulosique pendant au moins 24 heures pour produire de l'éthanol dans la biomasse cellulosique et générer une biomasse cellulosique ensilée, le prétraitement de la biomasse cellulosique ensilée avec une solution comprenant un acide alpha-hydroxysulfonique pour produire une matière prétraitée contenant de l'éthanol, et l'introduction d'une ou de plusieurs cellulases dans la matière prétraitée pour produire l'hydrolysat ; et (b) à fournir un réacteur de propagation contenant : un milieu de propagation comprenant l'hydrolysat en une quantité d'au moins 25 % en volume et du glucose en une quantité supérieure à 14 g/L ; et une première masse cellulaire d'un microorganisme de fermentation.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)