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1. (WO2017098206) EXTRACTION DISC
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/098206 International Application No.: PCT/GB2016/053732
Publication Date: 15.06.2017 International Filing Date: 28.11.2016
IPC:
C12Q 1/04 (2006.01) ,C12Q 1/18 (2006.01) ,C12Q 1/34 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
18
Testing for antimicrobial activity of a material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
Applicants:
MAST GROUP LIMITED [GB/GB]; Mast House Derby Road Liverpool Merseyside L20 1EA, GB
Inventors:
BLUNDELL, Jamie; GB
HOBSON, Jonathan Anthony; GB
Agent:
WP THOMPSON; 138 Fetter Lane London EC4A 1BT, GB
Priority Data:
1521899.311.12.2015GB
Title (EN) EXTRACTION DISC
(FR) DISQUE D'EXTRACTION
Abstract:
(EN) The present invention relates to a method for determining whether a microorganism produces a carbapenem or penem hydrolysing p-Iactamase, comprising: providing a reagent composition comprising a p-Iactam containing antibiotic, an extended-spectrum p-lactamase inhibitor and/or an AmpC inhibitor to which the carbapenem or penem hydrolysing p-lactamase is resistant, and a membrane permeabilizing agent which is ethylene glycol tetraacetic acid (EGTA) that permeabilizes the microorganism's outer membrane; contacting the microorganism suspected of producing a P-lactamase with the reagent composition; and culturing the microorganism with the reagent composition in a culture medium comprising a reporter microorganism wherein a positive test is determined by an increase in the growth of the reporter microorganism. The present invention also relates to an enzyme assay system and a kit for use in such a method.
(FR) La présente invention concerne une méthode permettant de déterminer si un microorganisme produit une β-lactamase hydrolysant les carbapénèmes ou les pénèmes, comprenant les étapes consistant : à fournir une composition de réactif comprenant un antibiotique contenant un β-lactame, un inhibiteur de la β-lactamase à spectre large et/ou un inhibiteur de l'AmpC auquel la β-lactamase hydrolysant les carbapénèmes ou les pénèmes est résistante, et un agent de perméabilisation de la membrane, lequel est de l'éthylène glycol tétraacétique (EGTA), qui perméabilise la membrane externe du microorganisme ; à mettre en contact les microorganismes suspectés de produire une β-lactamase avec la composition de réactif ; et à cultiver le microorganisme avec la composition de réactif dans un milieu de culture comprenant un microorganisme rapporteur, la positivité du test étant déterminée par l'augmentation de la croissance du microorganisme rapporteur. La présente invention concerne également un système de dosage enzymatique et un kit utilisé dans cette méthode.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP3387143