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1. (WO2017097887) METHODS AND KITS FOR JOINING FRAGMENTED NUCLEIC ACIDS TOGETHER
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/097887 International Application No.: PCT/EP2016/080225
Publication Date: 15.06.2017 International Filing Date: 08.12.2016
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
ROCHE DIAGNOSTICS GMBH [DE/DE]; Sandhofer Straße 116 68305 Mannheim, DE (DE)
F. HOFFMANN-LA ROCHE AG [CH/CH]; Grenzacherstrasse 124 4070 Basel, CH (AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BE, BF, BG, BH, BJ, BN, BR, BW, BY, BZ, CA, CF, CG, CH, CI, CL, CM, CN, CO, CR, CU, CY, CZ, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, FR, GA, GB, GD, GE, GH, GM, GN, GQ, GR, GT, GW, HN, HR, HU, ID, IE, IL, IN, IR, IS, IT, JP, KE, KG, KH, KM, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MC, MD, ME, MG, MK, ML, MN, MR, MT, MW, MX, MY, MZ, NA, NE, NG, NI, NL, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SI, SK, SL, SM, SN, ST, SV, SY, SZ, TD, TG, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, ZA, ZM, ZW)
ROCHE MOLECULAR SYSTEMS, INC. [US/US]; 4300 Hacienda Drive Pleasanton, California 94588, US (US)
Inventors:
WILL, Stephen G.; US
WANG, Yuker; US
Agent:
SCHWARZ, Ralf; DE
IMMENDOERFER-RUEHLE, Ulrike; DE
KNAUER, Martin; DE
LOHE, Hans-Joerg; DE
MUSSAUER, Heiko; DE
RAUSCHER, Andreas; DE
VON CAMPENHAUSEN, Harald; DE
HOEVELMANN, Sascha; DE
RIWOTZKI, Karsten; DE
KUNSTMANN, Tobias; DE
RITTER, Claudia; DE
MEINEL, Anne Julia; DE
MOLNAR, Ferenc; DE
Priority Data:
14/965,34310.12.2015US
Title (EN) METHODS AND KITS FOR JOINING FRAGMENTED NUCLEIC ACIDS TOGETHER
(FR) PROCÉDÉS ET KITS PERMETTANT DE JOINDRE DES ACIDES NUCLÉIQUES FRAGMENTÉS ENSEMBLE
Abstract:
(EN) Methods and kits for joining fragmented nucleic acid sequences together are provided, including performing an amplifying step including contacting a sample suspected of including a fragmented target nucleic acid with a pair of external primers and a pair of self- complementary internal primers, and generating a full length target nucleic acid. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, kits are provided that are designed for the detection of a target nucleic acid sequence.
(FR) La présente invention concerne des procédés et des kits permettant de joindre des séquences d'acides nucléiques fragmentées, comprenant la réalisation d'une étape d'amplification comportant la mise en contact d'un échantillon soupçonné de contenir un acide nucléique cible fragmenté avec une paire d'amorces externes et une paire d'amorces internes auto-complémentaires, et la génération d'un acide nucléique cible pleine longueur. Les procédés peuvent comprendre la réalisation d'une étape d'amplification, d'une étape d'hybridation et d'une étape de détection. En outre, des kits sont décrits, qui sont conçus pour la détection d'une séquence d'acide nucléique cible.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
CA3007447CN108291255EP3387146