Some content of this application is unavailable at the moment.
If this situation persists, please contact us atFeedback&Contact
1. (WO2017096617) REAGENT KIT FOR STEP-BY-STEP HUC-MSC CULTURE AND HUC-MSC ACQUIRED USING SAID REAGENT KIT
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/096617 International Application No.: PCT/CN2015/097150
Publication Date: 15.06.2017 International Filing Date: 11.12.2015
IPC:
C12N 5/0775 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775
Mesenchymal stem cells; Adipose-tissue derived stem cells
Applicants:
里程 LI, Cheng [CN/CN]; CN
郭镭 GUO, Lei [CN/CN]; CN
Inventors:
郭镭 GUO, Lei; CN
Agent:
北京瑞恒信达知识产权代理事务所(普通合伙) LEADING INTELLECTUAL PROPERTY FIRM; 中国北京市 海淀区大柳树路17号富海中心2号楼9层909房间 Room 909,9th Floor,Tower B,Fortune Building,No.17,Daliushu Road, Haidian District Beijing 100081, CN
Priority Data:
Title (EN) REAGENT KIT FOR STEP-BY-STEP HUC-MSC CULTURE AND HUC-MSC ACQUIRED USING SAID REAGENT KIT
(FR) TROUSSE DE RÉACTIF POUR CULTURE ÉTAPE PAR ÉTAPE DE HUC-MSC ET HUC-MSC ACQUISES AU MOYEN DUDIT KIT DE RÉACTIF
(ZH) 一种用于分步培养hUC-MSC的试剂盒及采用所述试剂盒获得的hUC-MSC
Abstract:
(EN) A serum-free culture method for human umbilical cord mesenchymal stem cells (hUC-MSC), said method using a step-by-step method to culture hUC-MSC: first using a TME culture medium for culturing for 3-4 hours to promote hUC-MSC adherence, and then switching to a TMD culture medium for rapid amplification.
(FR) L’invention concerne un procédé de culture sans sérum pour cellules souches mésenchymateuses de cordon ombilical humain (hUC-MSC), ledit procédé utilisant un procédé étape par étape de culture de hUC-MSC qui comprend les étapes qui consistent à : d’abord utiliser un milieu de culture TME pour une mise en culture pendant 3-4 heures afin de favoriser l’adhérence des hUC-MSC, puis à passer à un milieu de culture TMD pour l’amplification rapide.
(ZH) 一种人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUC-MSC)无血清培养方法,该方法采用分步法培养hUC-MSC:先使用TME培养基培养3-4小时促进hUC-MSC贴壁,然后更换为TMD培养基进行快速扩增。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)
Also published as:
US20180362932