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1. (WO2017096616) SERUM-FREE CULTURE MEDIUM AND PREPARATION METHOD AND APPLICATION THEREFOR
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/096616 International Application No.: PCT/CN2015/097148
Publication Date: 15.06.2017 International Filing Date: 11.12.2015
IPC:
C12N 5/0775 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775
Mesenchymal stem cells; Adipose-tissue derived stem cells
Applicants:
里程 LI, Cheng [CN/CN]; CN
郭镭 GUO, Lei [CN/CN]; CN
Inventors:
郭镭 GUO, Lei; CN
Agent:
北京瑞恒信达知识产权代理事务所(普通合伙) LEADING INTELLECTUAL PROPERTY FIRM; 中国北京市 海淀区大柳树路17号富海中心2号楼9层909房间 Room 909,9th Floor,Tower B,Fortune Building,No.17,Daliushu Road, Haidian District Beijing 100081, CN
Priority Data:
Title (EN) SERUM-FREE CULTURE MEDIUM AND PREPARATION METHOD AND APPLICATION THEREFOR
(FR) MILIEU DE CULTURE SANS SÉRUM ET PROCÉDÉ DE PRÉPARATION ET APPLICATION CORRESPONDANTS
(ZH) 一种无血清培养基及其制备方法和用途
Abstract:
(EN) Provided is a serum-free culture medium, the ingredients of the culture medium comprising 0.05-2 parts by volume of β-mercaptoethanol, 0.5-2 parts by volume of non-essential amino acid aqueous solution, 4-6 parts by volume of human mesenchymal stem cell culture supernatant concentrate, and 90-95 parts by volume of a-MEM/DMEM-F12 and recombinant human alkaline fibroblast growth factor of a final concentration of 5-5 ng/ml. The present culture medium is used for carrying out stem cell culture.
(FR) L'invention concerne un milieu de culture sans sérum, les ingrédients du milieu de culture comprenant 0,05-2 parties en volume de ß-mercaptoéthanol, 0,5-2 parties en volume d'une solution aqueuse d'acides aminés non essentiels, 4-6 parties en volume de concentrat de surnageant de culture de cellules souches mésenchymateuses humaines et 90-95 parties en volume d'a-MEM/DMEM-F12 et d'un facteur de croissance fibroblastique basique humain recombinant à une concentration finale de 5-5 ng/ml. Le milieu de culture selon l'invention est utilisé pour effectuer une culture de cellules souches.
(ZH) 提供一种新型无血清培养基,该培养基成分包括0.05-0.2体积份的β-巯基乙醇、0.5-2体积份的非必需氨基酸水溶液、4-6体积份的人间充质干细胞培养上清浓缩液、90-95体积份的a-MEM/DMEM-F12和终浓度为5-15ng/ml的重组人碱性成纤维细胞生长因子。该培养基用于进行干细胞培养。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)
Also published as:
US20180362920