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1. (WO2017096491) NUCLEIC ACID AMPLIFICATION BIOSENSOR FOR USE IN ROLLING CIRCLE AMPLIFICATION (RCA)
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/096491 International Application No.: PCT/CA2016/051458
Publication Date: 15.06.2017 International Filing Date: 12.12.2016
IPC:
C12Q 1/68 (2006.01) ,C07H 21/00 (2006.01) ,C12M 1/34 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
Applicants:
MCMASTER UNIVERSITY [CA/CA]; 1280 Main Street West Hamilton, Ontario L8S 4L8, CA
Inventors:
LI, Yingfu; CA
BRENNAN, John; CA
LIU, Meng; CA
Agent:
BERESKIN & PARR LLP/S.E.N.C.R.L., S.R.L.; 40 King Street West, 40th Floor Toronto, Ontario M5H 3Y2, CA
Priority Data:
62/266,22511.12.2015US
Title (EN) NUCLEIC ACID AMPLIFICATION BIOSENSOR FOR USE IN ROLLING CIRCLE AMPLIFICATION (RCA)
(FR) BIOCAPTEUR D'AMPLIFICATION D'ACIDE NUCLÉIQUE UTILE DANS L'AMPLIFICATION PAR CERCLE ROULANT (RCA)
Abstract:
(EN) The present application describes a biosensor for detecting target nucleic acid. The biosensor's mode of operation is based on binding of the target nucleic acid to another nucleic acid sequence and a circular template which triggers rolling circle amplification and detection of the amplified product as the indicator of the presence of the target nucleic acid. The biosensor is immobilized on a solid support, such as paper.
(FR) La présente invention concerne un biocapteur pour la détection d'un acide nucléique cible. Le mode de fonctionnement du biocapteur est fondé sur la liaison de l'acide nucléique cible à une autre séquence d'acide nucléique et à une matrice circulaire qui déclenche une amplification par cercle roulant et la détection du produit amplifié en tant qu'indicateur de la présence de l'acide nucléique cible. Le biocapteur est immobilisé sur un support solide, tel que du papier.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
CA3007848US20190024148