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1. (WO2017095917) DIGITAL MICROFLUIDIC SYSTEM FOR SINGLE-CELL ISOLATION AND CHARACTERIZATION OF ANALYTES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/095917 International Application No.: PCT/US2016/064206
Publication Date: 08.06.2017 International Filing Date: 30.11.2016
IPC:
C12Q 1/68 (2006.01) ,G01N 1/28 (2006.01) ,B01L 3/00 (2006.01) ,G01N 15/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
L
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3
Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15
Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10
Investigating individual particles
Applicants:
ILLUMINA, INC. [US/US]; 5200 Illumina, Way San Diego, CA 92122, US
Inventors:
JAMSHIDI, Arash; US
LIN, Yan-you; US
ABSALAN, Farnaz; US
STUART, Sarah; US
CANN, Gordon; US
WU, Yir-Shyuan; US
KHURANA, Tarun; US
FISHER, Jeffrey, S.; US
Agent:
SMALL, Dean, D.; US
Priority Data:
62/261,78601.12.2015US
62/281,51021.01.2016US
62/314,07128.03.2016US
62/358,96806.07.2016US
Title (EN) DIGITAL MICROFLUIDIC SYSTEM FOR SINGLE-CELL ISOLATION AND CHARACTERIZATION OF ANALYTES
(FR) SYSTÈME MICROFLUIDIQUE NUMÉRIQUE POUR L'ISOLEMENT DE CELLULES UNIQUES ET LA CARACTÉRISATION D'ANALYTES
Abstract:
(EN) In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
(FR) Des modes de réalisation de la présente invention concernent un procédé de capture de cellules d'intérêt dans un système microfluidique numérique comprenant l'utilisation d'un actionneur de gouttelettes pour transporter une gouttelette d'échantillon jusqu'à un dispositif à micropuits. Le dispositif à micropuits comprend un substrat pourvu d'une pluralité de micropuits qui s'ouvrent sur une surface d'opérations de gouttelettes du dispositif à micropuits. La gouttelette d'échantillon comprend des cellules d'intérêt qui entrent dans les micropuits. Le procédé consiste à introduire des billes de capture dans les micropuits, et les éléments de capture sont immobilisés sur les billes de capture. Le procédé utilise l'actionneur de gouttelettes pour transporter une gouttelette de réactif de lyse cellulaire jusqu'au dispositif à micropuits. Des parties de la gouttelette de réactif de lyse cellulaire entrent dans les micropuits et, pendant une période d'incubation, amènent les cellules d'intérêt à libérer l'analyte qui est capturé par les éléments de capture sur les billes de capture.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU2016364722CA3006985SG11201804563SUS20180250672CN108473927EP3384046