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1. (WO2017095333) A CELL CULTURE SUBSTRATE AND METHOD OF MAKING THEREOF
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/095333 International Application No.: PCT/SG2016/050587
Publication Date: 08.06.2017 International Filing Date: 30.11.2016
Chapter 2 Demand Filed: 22.06.2017
IPC:
C12N 5/0735 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
073
Embryonic cells or tissues; Foetal cells or tissues
0735
Embryonic stem cells; Embryonic germ cells
Applicants:
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis, Singapore 138632, SG
Inventors:
YING, Jackie Y.; SG
ERATHODIYIL, Nandanan; SG
NARAYANAN, Karthikeyan; SG
WAN, Andrew C. A.; SG
Agent:
SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office Singapore 903031, SG
Priority Data:
10201509845Q30.11.2015SG
Title (EN) A CELL CULTURE SUBSTRATE AND METHOD OF MAKING THEREOF
(FR) SUBSTRAT DE CULTURE CELLULAIRE ET PROCÉDÉ POUR SA FABRICATION
Abstract:
(EN) A cell culture substrate comprising a substrate having a coating of a plurality of amine functionalized nanoparticles is disclosed. In one embodiment, the amine functionalized nanoparticle is a polymer of an acrylamide monomer, a cross -linker and an amine monomer. There is also provided a method of making the cell culture substrate either by drying the amine functionalized nanoparticles when spread onto the said substrate or by covalent linkages of the substrate with thiol terminated nanoparticles. In addition, there is provided a method of culturing stem cells on the cell culture substrate having a coating of a plurality of the amine functionalized nanoparticles thereon.
(FR) L'invention concerne un substrat de culture cellulaire comprenant un substrat présentant un revêtement d'une pluralité de nanoparticules à fonction amine. Dans un mode de réalisation, la nanoparticule à fonction amine est un polymère d'un monomère de type acrylamide, d'un agent de réticulation et d'un monomère de type amine. L'invention concerne également un procédé de fabrication du substrat de culture cellulaire, soit par séchage des nanoparticules à fonction amine étalées sur ledit substrat, soit par des liaisons covalentes du substrat avec des nanoparticules à terminaison thiol. En outre, l'invention concerne un procédé de culture de cellules souches sur le substrat de culture cellulaire sur lequel se trouve un revêtement d'une pluralité de nanoparticules à fonction amine.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
SG11201804001RCN108350422US20180355310