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1. (WO2017093400) METHOD FOR DETERMINING CELL CLONALITY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/093400 International Application No.: PCT/EP2016/079427
Publication Date: 08.06.2017 International Filing Date: 01.12.2016
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
ARES TRADING S.A. [CH/CH]; Zone Industrielle de L'Ouriettaz 1170 Aubonne, CH
Inventors:
LA NEVE, Fabio; IT
FEGER, Georg; CH
TOSO, Emiliano; IT
Agent:
MERCK SERONO S.A.; Intellectual Property Terre Bonne Business Park A2 Route de Crassier 15 1162 Eysins, CH
Priority Data:
15197894.703.12.2015EP
Title (EN) METHOD FOR DETERMINING CELL CLONALITY
(FR) MÉTHODE DE DÉTERMINATION DE LA CLONALITÉ CELLULAIRE
Abstract:
(EN) The invention relates to a method for determining the clonality of a master cell bank (MCB) in which a transgene has been inserted. The method involves a combination of sequencing methodology and bioinformatic analysis, performed on multiple subclones originating from a common MCB, to establish a reliable set of reference transgene insertion regions in one reference subclone, and corresponding sets of comparative transgene insertion regions in one or more other subclones originating from the same MCB. Based on the degree of congruity between reference and comparative transgene insertion regions, the MCB is determined to be either monoclonal or polyclonal.
(FR) L'invention concerne une méthode de détermination de la clonalité d'une banque cellulaire maîtresse MCB dans laquelle a été inséré un transgène. La méthode implique de combiner une méthodologie de séquençage et une analyse bioinformatique, exécutées sur de nombreux sous-clones provenant d'une MCB commune, afin d'établir un ensemble fiable de régions de référence d'insertion du transgène dans un sous-clone de référence, et d'ensembles correspondants de régions d'insertion du transgène comparées, situées dans un ou plusieurs autres sous-clones provenant de la même MCB. Sur la base du degré de congruence entre des régions d'insertion du transgène comparées et de référence, la MCB est définie comme étant monoclonale ou polyclonale.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU2016363746CA3005386CN108368549KR1020180088867EP3384045US20180327830
IL259750