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1. (WO2017092204) METHOD FOR CONSTRUCTING SECOND-GENERATION SEQUENCING LIBRARY FOR DETECTION OF LOW-FREQUENCY MUTATION, AND KIT THEREFOR
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/092204 International Application No.: PCT/CN2016/077492
Publication Date: 08.06.2017 International Filing Date: 28.03.2016
IPC:
C12N 15/10 (2006.01) ,C40B 50/06 (2006.01) ,C40B 40/06 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
50
Methods of creating libraries, e.g. combinatorial synthesis
06
Biochemical methods, e.g. using enzymes or whole viable micro-organisms
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40
Libraries per se, e.g. arrays, mixtures
04
Libraries containing only organic compounds
06
Libraries containing nucleotides or polynucleotides, or derivatives thereof
Applicants:
南京安诺优达基因科技有限公司 NANJING ANNOROAD GENE TECHNOLOGY CO. LTD [CN/CN]; CN
安诺优达基因科技(北京)有限公司 ANNOROAD GENE TECHNOLOGY (BEIJING) CO., LTD [CN/CN]; 中国北京市 北京经济技术开发区科创六街88号院8号楼B2栋 Building B2 Yard 88, Kechuang 6 Rd., Beijing Economic-Technological Development Area Beijing 100176, CN
Inventors:
王秀莉 WANG, Xiuli; CN
王旺 WANG, Wang; CN
荆瑞林 JING, Ruilin; CN
玄兆伶 XUAN, Zhaoling; CN
李大为 LI, Dawei; CN
梁峻彬 LIANG, Junbin; CN
陈重建 CHEN, Chongjian; CN
Agent:
北京坤瑞律师事务所 WU, FENG & ZHANG; 中国北京市 海淀区海淀大街8号中钢国际广场A座21层A/D区 Sinosteel Plaza, 21st Floor A/D, 8 Haidian st., Haidian Beijing 100080, CN
Priority Data:
201510857393.230.11.2015CN
Title (EN) METHOD FOR CONSTRUCTING SECOND-GENERATION SEQUENCING LIBRARY FOR DETECTION OF LOW-FREQUENCY MUTATION, AND KIT THEREFOR
(FR) PROCÉDÉ DE CONSTRUCTION D'UNE BANQUE DE SÉQUENÇAGE DE SECONDE GÉNÉRATION POUR LA DÉTECTION D'UNE MUTATION À BASSE FRÉQUENCE, ET KIT ASSOCIÉ
(ZH) 用于低频突变检测的二代测序文库的构建方法及试剂盒
Abstract:
(EN) The present invention provides a method for constructing a second-generation sequencing library for the detection of a low-frequency mutation, and a kit therefor. The method comprises: obtaining a DNA fragment with blunt ends, obtaining a DNA fragment added with A at the 3'-terminal end, obtaining a DNA fragment added with an adapter using particular nucleotide sequences, and obtaining an amplification product using particular nucleotide sequences.
(FR) La présente invention concerne un procédé de construction d'une banque de séquençage de seconde génération pour la détection d'une mutation à basse fréquence, et un kit associé. Le procédé consiste : à obtenir un fragment d'ADN à extrémités franches, à obtenir un fragment d'ADN ayant un A ajouté au niveau de l'extrémité terminale 3', à obtenir un fragment d'ADN ayant un adaptateur ajouté à l'aide de séquences nucléotidiques particulières, et à obtenir un produit d'amplification à l'aide de séquences nucléotidiques particulières.
(ZH) 本发明提供一种用于低频突变检测的二代测序文库构建方法及试剂盒。所述构建方法包括得到平末端DNA片段、得到3'端加A的DNA片段、使用特定核苷酸序列得到加接头DNA片段以及使用特定核苷酸序列得到扩增产物等步骤。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)
Also published as:
EP3385379US20190017044