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1. (WO2017090761) METHOD FOR CONVERTING MONOCOT PLANT GENOME SEQUENCE IN WHICH NUCLEIC ACID BASE IN TARGETED DNA SEQUENCE IS SPECIFICALLY CONVERTED, AND MOLECULAR COMPLEX USED THEREIN
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/090761 International Application No.: PCT/JP2016/085075
Publication Date: 01.06.2017 International Filing Date: 25.11.2016
IPC:
C12N 15/09 (2006.01) ,C12N 9/10 (2006.01) ,C12N 9/16 (2006.01) ,C12N 9/78 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10
Transferases (2.)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
78
acting on carbon to nitrogen bonds other than peptide bonds (3.5)
Applicants:
国立大学法人神戸大学 NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY [JP/JP]; 兵庫県神戸市灘区六甲台町1-1 1-1, Rokkodai-cho, Nada-ku, Kobe-shi, Hyogo 6578501, JP
Inventors:
西田 敬二 NISHIDA, Keiji; JP
島谷 善平 SHIMATANI, Zenpei; JP
近藤 昭彦 KONDO, Akihiko; JP
Agent:
高島 一 TAKASHIMA, Hajime; JP
Priority Data:
2015-23237927.11.2015JP
2016-13461306.07.2016JP
Title (EN) METHOD FOR CONVERTING MONOCOT PLANT GENOME SEQUENCE IN WHICH NUCLEIC ACID BASE IN TARGETED DNA SEQUENCE IS SPECIFICALLY CONVERTED, AND MOLECULAR COMPLEX USED THEREIN
(FR) PROCÉDÉ DE CONVERSION DE SÉQUENCE DE GÉNOME DE MONOCOTYLÉDONE CONVERTISSANT SPÉCIFIQUEMENT UNE BASE D’ACIDE NUCLÉIQUE D’UNE SÉQUENCE ADN CIBLÉE, ET COMPLEXE MOLÉCULAIRE METTANT EN ŒUVRE CELUI-CI
(JA) 標的化したDNA配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体
Abstract:
(EN) The present invention provides a method for modifying a targeted site in double-stranded DNA possessed by monocot plant cells, which includes a step for, by bringing a complex, in which a nucleic acid sequence-recognizing module that specifically bonds with a target nucleotide sequence in a selected double-stranded DNA is bonded with a nucleic acid base converting enzyme, into contact with the double-stranded DNA, converting one or more nucleotides at the targeted site to one or more different nucleotides, deleting one or more nucleotides at the targeted site, or inserting one or more nucleotides to the targeted site, without cleaving at least one of the strands of the double-stranded DNA at the targeted site, wherein the contact between the double-stranded DNA and the complex is achieved by introducing a nucleic acid coding for the complex to the monocot plant cells. In addition, also provided is the complex to be used in this method, in which the nucleic acid sequence-recognizing module that specifically bonds with a target nucleotide sequence in a double-stranded DNA possessed by monocot plant cells is bonded with a nucleic acid base converting enzyme.
(FR) L’invention concerne un procédé qui convertit une région ciblée d’un ADN double brin possédant une cellule de monocotylédone. Ce procédé inclut une étape au cours de laquelle un complexe dans lequel sont liés un module de reconnaissance d’acide nucléique spécifiquement lié à une séquence de nucléotides cible dans l’ADN double brin choisi, et un enzyme de conversion de base d’acide nucléique, est mis en contact avec cet ADN double brin, au moins un brin de cet ADN double brin est coupé dans la région ciblée, et un nucléotide ou plus de la région ciblée est converti en un autre nucléotide ou plus, est soumis à une délétion, ou un nucléotide ou plus est introduit dans la région ciblée. En outre, l’invention fournit un complexe dans lequel sont liés un module de reconnaissance d’acide nucléique spécifiquement lié à une séquence de nucléotides cible dans l’ADN double brin possédant une cellule de monocotylédone, et un enzyme de conversion de base d’acide nucléique, afin de le mettre en œuvre dans le procédé de l’invention.
(JA) 本発明は、単子葉植物細胞の有する二本鎖DNAの標的化された部位を改変する方法であって、選択された二本鎖DNA中の標的ヌクレオチド配列と特異的に結合する核酸配列認識モジュールと、核酸塩基変換酵素とが結合した複合体を、該二本鎖DNAと接触させ、該標的化された部位において該二本鎖DNAの少なくとも一方の鎖を切断することなく、該標的化された部位の1以上のヌクレオチドを他の1以上のヌクレオチドに変換する又は欠失させる、あるいは該標的化された部位に1以上のヌクレオチドを挿入する工程を含み、該二本鎖DNAと該複合体との接触が、該単子葉植物細胞への該複合体をコードする核酸の導入により行われる、方法を提供する。さらに、当該方法に使用するための、単子葉植物細胞の有する二本鎖DNA中の標的ヌクレオチド配列と特異的に結合する核酸配列認識モジュールと、核酸塩基変換酵素とが結合した複合体も提供する。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
KR1020180081811CN108495932EP3382019BR112018010681US20190085342