Search International and National Patent Collections
Some content of this application is unavailable at the moment.
If this situation persists, please contact us atFeedback&Contact
1. (WO2017090543) METHOD FOR ANALYZING INTER-DNA INTERACTION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2017/090543 International Application No.: PCT/JP2016/084358
Publication Date: 01.06.2017 International Filing Date: 18.11.2016
IPC:
C12Q 1/68 (2006.01) ,C12N 15/09 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
Applicants:
国立大学法人大阪大学 OSAKA UNIVERSITY [JP/JP]; 大阪府吹田市山田丘1番1号 1-1, Yamadaoka, Suita-shi, Osaka 5650871, JP
Inventors:
藤井 穂高 FUJII Hodaka; JP
藤田 敏次 FUJITA Toshitsugu; JP
Agent:
岩谷 龍 IWATANI, Ryo; JP
Priority Data:
2015-23132927.11.2015JP
Title (EN) METHOD FOR ANALYZING INTER-DNA INTERACTION
(FR) PROCÉDÉ D'ANALYSE D'INTERACTION ENTRE ADN
(JA) DNA間相互作用の解析方法
Abstract:
(EN) Provided is a method for analyzing inter-DNA interaction comprising: (1) a step for preparing a fragmented DNA by ultrasonication or by a partial decomposition process using an endonuclease without DNA sequence specificity, the DNA to be fragmented being in a condition in which the DNA to be analyzed and another DNA are interacting with each other; (2) a step for ligating the fragmented DNA; (3) a step for, from ligated DNA, amplifying and/or purifying a composite containing the DNA to be analyzed and the DNA interacting with said DNA; and (4) a step for analyzing interacting DNA in the amplified and/or purified composite. The method does not require costly equipment or special skills and can be easily implemented by any researcher. Furthermore, the method for analyzing inter DNA-interaction obtains highly reliable results and can be implemented without the use of a restriction enzyme.
(FR) L'invention concerne un procédé d'analyse d'interaction entre ADN, lequel comporte: (1) une étape de préparation d'ADN fragmenté par ultrasonication ou par décomposition partielle à l'aide d'une endonucléase ne présentant pas de spécificité de séquence d'ADN, cet ADN fragmenté contenant un ADN dans lequel l'ADN cible et un autre ADN interagissent; (2) une étape de ligature de l'ADN fragmenté; (3) une étape d'amplification et/ou de purification d'un composite provenant de l'ADN après ligature et contenant l'ADN cible et un ADN interagissant avec l'ADN cible; et (4) une étape d'analyse des ADN qui interagissent dans le composite amplifié et/ou purifié. Ce procédé ne nécessite ni appareil coûteux, ni compétence spécifique et peut être mis en oeuvre facilement par un chercheur. De plus, ce procédé d'analyse d'interaction entre ADN permet d'obtenir des résultats d'une grande fiabilité et peut être appliqué sans l'utilisation d'enzyme de restriction.
(JA) (1)解析対象DNAと他のDNAとが相互作用している状態のDNAを含み、超音波処理またはDNA配列特異性を持たないエンドヌクレアーゼによる部分分解処理により断片化DNAを調製する工程、(2)断片化DNAのライゲーションを行う工程、(3)ライゲーション後のDNAから、解析対象DNAおよび当該DNAと相互作用しているDNAを含む複合体を増幅および/または精製する工程、ならびに(4)増幅および/または精製された複合体中の前記相互作用しているDNAを解析する工程を含むDNA間相互作用の解析方法を提供する。本方法は、高価な機器や熟練を必要とせず、一般の研究者が簡便に実施することができ、信頼性の高い結果を得ることができるDNA間相互作用の解析方法であり、かつ、制限酵素を用いずに実施できるDNA間相互作用の解析方法である。
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)