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1. (WO2017070633) EVOLVED CAS9 PROTEINS FOR GENE EDITING

Pub. No.:    WO/2017/070633    International Application No.:    PCT/US2016/058345
Publication Date: Fri Apr 28 01:59:59 CEST 2017 International Filing Date: Sun Oct 23 01:59:59 CEST 2016
IPC: C07K 14/32
C12N 9/22
C12N 9/78
C12N 15/90
C12N 15/10
Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Inventors: LIU, David, R.
HU, Johnny, Hao
Title: EVOLVED CAS9 PROTEINS FOR GENE EDITING
Abstract:
Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for enginerring Cas9 and Cas9 variants that have increased activity on target sequences that do not contain the canonical PAM sequence. In some embodiments, fusion proteins comprising such Cas9 variants and nucleic acid editing domains, e.g., deaminase domains, are provided.