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1. (WO2017069829) HIGH-THROUGHPUT STRATEGY FOR DISSECTING MAMMALIAN GENETIC INTERACTIONS

Pub. No.:    WO/2017/069829    International Application No.:    PCT/US2016/044969
Publication Date: Fri Apr 28 01:59:59 CEST 2017 International Filing Date: Tue Aug 02 01:59:59 CEST 2016
IPC: C12N 15/10
C12N 15/63
C12N 15/85
C12Q 1/68
Applicants: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Inventors: WANG, Harris
SHAPIRA, Sagi
STOCKMAN, Victoria
Title: HIGH-THROUGHPUT STRATEGY FOR DISSECTING MAMMALIAN GENETIC INTERACTIONS
Abstract:
The present disclosure provides for a rapid and systematic method to map genetic interactions using the CRISPR/Cas system. A set of genes, such as the entire genome of an organism, can he targeted in pairs by a CRISPR guide RNA library. Each vector of the library contains at least two CRISPR guide sequences which encode gRNAs, The library can target all genes of the set in a pairwise fashion.