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1. (WO2017069563) DNA PURIFYING APPARATUS AND DNA PURIFYING METHOD
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2017/069563 International Application No.: PCT/KR2016/011902
Publication Date: 27.04.2017 International Filing Date: 21.10.2016
IPC:
C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 35/00 (2006.01) ,G01N 35/08 (2006.01) ,B01L 3/00 (2006.01)
Applicants: UNIST(ULSAN NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY)[KR/KR]; 50, UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan 44919, KR
Inventors: CHO, Yoon Kyoung; KR
KIM, Chi Ju; KR
KIM, Tae Hyeong; KR
Agent: YOU ME PATENT AND LAW FIRM; 115 Teheran-ro Gangnam-gu Seoul 06134, KR
Priority Data:
10-2015-014756522.10.2015KR
Title (EN) DNA PURIFYING APPARATUS AND DNA PURIFYING METHOD
(FR) APPAREIL DE PURIFICATION D'ADN ET PROCÉDÉ DE PURIFICATION D'ADN
(KO) 핵산 정제 장치 및 핵산 정제 방법
Abstract: front page image
(EN) Provided is a DNA purifying apparatus comprising: a disc rotatably provided by using a centrifugal force-based disc-shaped chip, such that the entire process of purifying cell-free DNA in a large amount of blood and in body fluid can be integrated, so as to allow fluid to be transferred by a centrifugal force; a supply part provided at the disc so as to supply a sample and a reagent, which is necessary for DNA purification; an adsorption reaction chamber provided at the disc, connected to the supply part, and accommodating, thereinside, an adsorption medium for DNA adsorption such that DNA is adsorbed from the sample; and a solution accommodation part connectively provided to the outlet side of the adsorption reaction part in the centrifugal direction of the disc, so as to accommodate a solution, which passes through the adsorption reaction chamber by a centrifugal force so as to be discharged, and discharging the same to the outside.
(FR) L'invention concerne un appareil de purification d'ADN comprenant : un disque entrant en rotation en utilisant une plaquette en forme de disque sur la base d'une force centrifuge, de façon à pouvoir intégrer la totalité du procédé de purification de l'ADN exempt de cellules dans une grande quantité de sang et dans un liquide corporel, de manière à pouvoir transférer un fluide par force centrifuge ; une partie d'alimentation disposée sur le disque de manière à fournir un échantillon et un réactif, qui est nécessaire pour la purification de l'ADN ; une chambre de réaction d'adsorption prévue sur le disque, reliée à la partie d'alimentation, et recevant, en son sein, un milieu d'adsorption pour l'adsorption de l'ADN de sorte que l'ADN provenant de l'échantillon est adsorbé ; et une partie de réception de solution disposée en raccordement vers le côté sortie de la partie de réaction d'adsorption dans la direction centrifuge du disque, de manière à recevoir une solution, qui passe à travers la chambre de réaction d'adsorption par force centrifuge de façon à être évacuée, et à l'évacuer vers l'extérieur.
(KO) 원심력을 기반으로 하는 디스크 모양의 칩을 활용하여, 다량의 혈액 및 체액으로부터 세포-자유 핵산 정제 전 과정이 일체화될 수 있도록, 회전가능하게 설치되어 원심력에 의한 유체의 이송이 이루어지는 디스크, 상기 디스크에 설치되어 시료 및 핵산 정제에 필요한 시약을 공급하기 위한 공급부, 상기 디스크에 설치되고 상기 공급부와 연결되며 내부에는 핵산 흡착을 위한 흡착 매개체가 수용되어 시료로부터 핵산 흡착이 이루어지는 흡착 반응챔버, 및 디스크의 원심력 방향을 따라 상기 흡착 반응부 출측에 연결 설치되어 원심력에 의해 흡착 반응챔버를 통과하여 배출되는 용액을 수용하며 외부로 배출하는 용액 수용부를 포함하는 핵산 정제 장치를 제공하고자 한다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)