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|1. (WO2017068801) EVALUATION METHOD FOR DIFFERENTIATION STATE OF CELLS|
TOKYO ELECTRON LIMITED
|Title:||EVALUATION METHOD FOR DIFFERENTIATION STATE OF CELLS|
The present invention is a method for evaluating the differentiation state of test cells on the basis of the amount of a prescribed indicator substance that is present on a culture supernatant for the test cells, which are stem cells that have an unknown differentiation state or cells that have been induced to differentiate from stem cells. The indicator substance is at least one compound selected from the group that consists of ornithine, 2-aminoadipic acid, deoxycytidine, glutamic acid, tryptophan, asparagine, alanine, cystine, hypoxanthine, uridine, aspartic acid, arginine, 2-hydroxybutyric acid, 2-hydroxyisovalerate, 3-hydroxyisovalerate, urea, 4-hydroxybenzoic acid, 4-aminobenzoic acid, ribonic acid, kynurenine, cystathionine, threonic acid, pyruvic acid, putrescine, ascorbic acid, riboflavin, serine, cysteine, orotic acid, and citric acid. According to one embodiment, differentiation state is evaluated on the basis of the amount of two or more indicator substances that is present.