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1. (WO2017068801) EVALUATION METHOD FOR DIFFERENTIATION STATE OF CELLS

Pub. No.:    WO/2017/068801    International Application No.:    PCT/JP2016/058303
Publication Date: Fri Apr 28 01:59:59 CEST 2017 International Filing Date: Thu Mar 17 00:59:59 CET 2016
IPC: C12Q 1/02
C12N 5/071
C12N 5/0735
C12N 5/10
Applicants: SHIMADZU CORPORATION
株式会社島津製作所
TOKYO ELECTRON LIMITED
東京エレクトロン株式会社
Inventors: SUZUKI, Takashi
鈴木 崇
HIRAMARU, Daisuke
平丸 大介
TAKAHASHI, Masatoshi
高橋 雅俊
TOMITA, Kentaro
富田 健太郎
HATABAYASHI, Kunitada
畑林 邦忠
KAGAWA, Kenichi
加川 健一
OZAKI, Shigenori
尾▲崎▼ 成則
Title: EVALUATION METHOD FOR DIFFERENTIATION STATE OF CELLS
Abstract:
The present invention is a method for evaluating the differentiation state of test cells on the basis of the amount of a prescribed indicator substance that is present on a culture supernatant for the test cells, which are stem cells that have an unknown differentiation state or cells that have been induced to differentiate from stem cells. The indicator substance is at least one compound selected from the group that consists of ornithine, 2-aminoadipic acid, deoxycytidine, glutamic acid, tryptophan, asparagine, alanine, cystine, hypoxanthine, uridine, aspartic acid, arginine, 2-hydroxybutyric acid, 2-hydroxyisovalerate, 3-hydroxyisovalerate, urea, 4-hydroxybenzoic acid, 4-aminobenzoic acid, ribonic acid, kynurenine, cystathionine, threonic acid, pyruvic acid, putrescine, ascorbic acid, riboflavin, serine, cysteine, orotic acid, and citric acid. According to one embodiment, differentiation state is evaluated on the basis of the amount of two or more indicator substances that is present.