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1. (WO2017066663) METHOD OF MANUFACTURING CELLS USING A HOLLOW FIBER BIOREACTOR

Pub. No.:    WO/2017/066663    International Application No.:    PCT/US2016/057172
Publication Date: Fri Apr 21 01:59:59 CEST 2017 International Filing Date: Sat Oct 15 01:59:59 CEST 2016
IPC: C12M 1/12
C12N 5/0735
C12N 5/074
C12N 5/0793
C12N 5/0797
C12N 5/077
C12N 5/0775
Applicants: STEMONIX INC.
Inventors: CARROMEU, Cassiano
SI, Wonjong
SPANGENBERG, Stephan
PETCAVICH, Robert John
Title: METHOD OF MANUFACTURING CELLS USING A HOLLOW FIBER BIOREACTOR
Abstract:
The present disclosure provides a method of manufacturing and differentiating mammalian stem cells, and in one embodiment human induced pluripotent stem cells (iPSc), e.g., manufacturing neuron progenitors, e.g., derived from iPSC, on a large scale by the use of an automated hollow fiber reactor system. In one embodiment, human iPSc that can be differentiated into cardiomyocytes or neuron progenitors are provided. The method comprises seeding a hollow fiber reactor with stem cells such as iPSc, or differentiated iPSc, growing and expanding the seeded cells using appropriate growth factors and nutrients, and harvesting the cells after expansion from the hollow fiber reactor walls, e.g., with the use of an enzyme. The method can produce billions of cells per week from seeding the reactor with a minimum number of starting stem cells or neuron progenitor cells.