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1. (WO2017065483) METHOD FOR INCREASING PURIFICATION YIELD OF NODAVIRUS CAPSID PROTEIN
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2017/065483    International Application No.:    PCT/KR2016/011393
Publication Date: 20.04.2017 International Filing Date: 12.10.2016
IPC:
C07K 1/22 (2006.01), B01D 15/38 (2006.01), C07K 14/08 (2006.01)
Applicants: CHUNG-ANG UNIVERSITY INDUSTRY-ACADEMY COOPERATION FOUNDATION [KR/KR]; 84, Heukseok-ro Dongjak-gu Seoul 06974 (KR)
Inventors: KIM, Hong Jin; (KR).
KIM, Hyoung Jin; (KR).
KWAK, Hye Lim; (KR)
Agent: TAEBAEK INTELLECTUAL PROPERTY LAW FIRM; #601 Innoplex 1 cha, 151Gasandigital 1-ro Geumcheon-gu Seoul 08506 (KR)
Priority Data:
10-2015-0142062 12.10.2015 KR
10-2016-0131818 12.10.2016 KR
Title (EN) METHOD FOR INCREASING PURIFICATION YIELD OF NODAVIRUS CAPSID PROTEIN
(FR) PROCÉDÉ D'AUGMENTATION DU RENDEMENT DE PURIFICATION DE PROTÉINE DE CAPSIDE DE NODAVIRUS
(KO) 노다바이러스 캡시드 단백질의 정제 수율 증가 방법
Abstract: front page image
(EN)The present invention relates to a method for increasing the purification yield of a nodavirus capsid protein. According to the present invention, in the purification procedure of RGNNV capsid protein, the dialysis of cell lysates before a chromatography step with a buffer containing CaCl2 or CaCl2 and EDTA can significantly increase the yield of the RGNNV capsid protein recovered at the time of chromatography. According to the present invention, the dialysis using a buffer containing only EDTA may diversify the size of the RGNNV capsid protein complex to lower the homogeneity or reduce the purification yield in the chromatography step, but the simultaneous treatment with EDTA and CaCl2 can remarkably increase the purification yield in the chromatography step. In addition, the degree of improvement in the purification yield by the simultaneous treatment with CaCl2 and EDTA is significantly higher than the level of improvement in the purification yield shown in the treatment condition with only CaCl2.
(FR)La présente invention concerne un procédé permettant d'augmenter le rendement de purification d'une protéine de capside de nodavirus. Selon la présente invention, dans le procédé de purification de protéine de capside RGNNV, la dialyse de lysats cellulaires avant une étape de chromatographie à l'aide d'un tampon contenant du CaCl2 ou du CaCl2 et de l'EDTA permet d'augmenter de façon significative le rendement de la protéine de capside RGNNV récupérée au moment de la chromatographie. Selon la présente invention, la dialyse à l'aide d'un tampon ne contenant que de l'EDTA peut diversifier la taille du complexe de protéine de capside RGNNV jusqu'abaisser l'homogénéité ou réduire le rendement de purification à l'étape de chromatographie, mais le traitement simultané avec de l'EDTA et du CaCl2 permet d'augmenter remarquablement le rendement de purification à l'étape de chromatographie. De plus, le degré d'amélioration du rendement de purification par le traitement simultané avec du CaCl2 et de l'EDTA est significativement plus élevé que le niveau d'amélioration du rendement de purification obtenu lors d'un traitement avec uniquement du CaCl2.
(KO)본 발명은 노다바이러스 캡시드 단백질의 정제 수율 증가 방법에 관한 것이다. 본 발명에 따르면, RGNNV 캡시드 단백질의 정제과정에서 크로마토그래피 단계 전 세포파쇄물을 CaCl2 또는 CaCl2와 EDTA가 포함된 완충액으로 투석할 경우 크로마토그래피시 회수되는 RGNNV 캡시드 단백질의 수율을 현격하게 증가시킬 수 있다. 본 발명에 따르면, EDTA만을 완충액에 포함시켜 투석을 진행 할 경우 RGNNV 캡시드 단백질 복합체의 크기를 다양하게 하여 균질성을 떨어뜨리거나 크로마토그래피 단계에서 정제 수율 감소를 일으킬 수 있으나, EDTA를 CaCl2와 동시에 처리할 경우 크로마토그래피 단계에서 정제 수율은 현격하게 증가될 수 있다. 또한 CaCl2와 EDTA를 동시에 처리함으로 인해 증가되는 정제 수율의 향상 정도는, CaCl2만 처리한 조건에서 보여지는 정제 수율 향상 수준보다 월등하게 높다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)