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1. (WO2017063787) MUTANT BETA-GLUCOSIDASE VARIANTS WITH INCREASED THERMOSTABILITY

Pub. No.:    WO/2017/063787    International Application No.:    PCT/EP2016/070804
Publication Date: Fri Apr 21 01:59:59 CEST 2017 International Filing Date: Tue Sep 06 01:59:59 CEST 2016
IPC: C12N 9/42
Applicants: TECHNISCHE UNIVERSITAET MUENCHEN
Inventors: ZVERLOV, Vladimir
SCHWARZ, Wolfgang
PRECHTL, Roman
LEIS, Benedikt
HELD, Claudia
LIEBL, Wolfgang
Title: MUTANT BETA-GLUCOSIDASE VARIANTS WITH INCREASED THERMOSTABILITY
Abstract:
The invention relates to mutant variants of the β-glucosidase Cgl T from Thermoanaerobacter brockii and nucleic acids for producing the same. Said mutant variants show significantly increased thermostability and enzyme activity. Furthermore, the invention provides vectors, host cells and methods for producing said mutant variants of the β-glucosidase Cgl T. Also provided are artificial cellulosomes comprising the mutant variants of the β-glucosidase Cgl T and methods for the enzymatic hydrolysis of cellulosic biomass comprising said artificial cellulosomes and/or said mutant variants of the β-glucosidase Cgl T.