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1. (WO2017060385) FLUORESCENCE MICROSCOPE INSTRUMENT COMPRISING AN ACTIVELY SWITCHED BEAM PATH SEPARATOR

Pub. No.:    WO/2017/060385    International Application No.:    PCT/EP2016/073928
Publication Date: Fri Apr 14 01:59:59 CEST 2017 International Filing Date: Fri Oct 07 01:59:59 CEST 2016
IPC: G02B 21/00
G02B 27/28
Applicants: DEUTSCHES KREBSFORSCHUNGSZENTRUM
Inventors: ENGELHARDT, Johann
Title: FLUORESCENCE MICROSCOPE INSTRUMENT COMPRISING AN ACTIVELY SWITCHED BEAM PATH SEPARATOR
Abstract:
A fluorescence microscope instrument (1) includes a light source (2) providing light (3) to be directed to a sample (4); a detector (5) detecting fluorescence light (5) emitted out of the sample (4); an objective (7) focusing the light (3) from the light source (2) into a focal area within the sample (4) and collecting the fluorescence light (6) emitted out of the focal area to be detected by the detector (5); and a beam path separator (9) arranged in a beam path of the light (3) from the light source (2) between the light source (2) and the objective (7) and in a beam path of the fluorescence light (6) between the objective (7) and the detector (5). Wavelengths of the light (3) to be directed to the sample (4) and of the light (6) to be detected by the detector fall into a range of wavelengths extending from a low end wavelength over at least 20 % of the low end wavelength. The beam path separator (9) separates the beam path of the fluorescence light (6) from the beam path of the light (3) from the light source (2) in that it is transferable between a first state in which it is transparent for light of any wavelength falling in the range of wavelengths and coming along the beam path of the light (3) from the light source (2), and a second state in which it is transparent for light of any wavelength falling in the range of wavelengths and coming along the beam path of the fluorescence light (6) from the sample (4).