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1. (WO2017058884) MODIFIED BACTERIAL PROTEIN EXPRESSION SYSTEM
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.:    WO/2017/058884    International Application No.:    PCT/US2016/054130
Publication Date: 06.04.2017 International Filing Date: 28.09.2016
IPC:
C12P 21/04 (2006.01), C12P 21/02 (2006.01), C12N 15/63 (2006.01), C12N 15/52 (2006.01), C12N 15/65 (2006.01), C12N 15/67 (2006.01), C12N 15/70 (2006.01)
Applicants: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK [US/US]; West 116th Street and Broadway New York, NY 10027 (US)
Inventors: GOTTESMAN, Maxwell; (US)
Agent: DAVITZ, Michael, A.; (US).
DAVITZ, Michael, A.; (US).
LEASON, David; (US).
CHENG, Susie; (US).
ELLIS, Edward, J.; (US)
Priority Data:
62/233,507 28.09.2015 US
Title (EN) MODIFIED BACTERIAL PROTEIN EXPRESSION SYSTEM
(FR) SYSTÈME D'EXPRESSION DE PROTÉINES BACTÉRIENNES MODIFIÉES
Abstract: front page image
(EN)The present disclosure provides host cells for reliable., high yield recombinant protein production, including unstable proteins. The present host cell (e.g., a bacterial cell) is deficient in at least one protease (or a subunit of a protease) such as Clp or ClpP. The host cell may also contain an expression vector that encodes a protein or polypeptide for overexpression.
(FR)La présente invention concerne des cellules hôtes pour une production fiable, à rendement élevé, de protéines recombinées, comprenant des protéines instables. La cellule hôte selon l'invention (par exemple, une cellule bactérienne) présente un déficit en au moins une protéase (ou un sous-motif d'une protéase) telle que Clp ou ClpP. Ladite cellule hôte peut également contenir un vecteur d'expression qui code pour une protéine ou un polypeptide destiné à être sur-exprimé.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)