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1. (WO2017040950) METHODS FOR DIRECT MEASUREMENT OF MICRORNA INHIBITION AND MIMICRY

Pub. No.:    WO/2017/040950    International Application No.:    PCT/US2016/050136
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Sat Sep 03 01:59:59 CEST 2016
IPC: C12Q 1/68
G01N 33/50
Applicants: REGULUS THERAPEUTICS INC.
Inventors: ANDROSAVICH, John R.
CHAU, B. Nelson
GALASSO, Ryan R.
Title: METHODS FOR DIRECT MEASUREMENT OF MICRORNA INHIBITION AND MIMICRY
Abstract:
The disclosure includes methods of determining the activity of an inhibitor of a target microRNA (miRNA) or of a mimic of a target microRNA. Determination of polysome occupancy in treated and control samples followed by comparing the occupancies can be used to determine a displacement value for the inhibitor or mimic of the target miRNA. The displacement value reflects the extent of a change in the levels of the target miRNA (which may include the mimic if applicable) in polysomal and non-polysomal compartments of a sample or a shift of the target miRNA between polysomal and non-polysomal compartments of a sample and can indicate the activity of the inhibitor toward the target miRNA or of the mimic toward a target RNA.