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1. (WO2017040859) METHODS AND COMPOSITIONS FOR MODULATING PRFA MEDIATED VIRULENCE GENE ACTIVATION IN LISTERIA SPP.

Pub. No.:    WO/2017/040859    International Application No.:    PCT/US2016/050004
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Fri Sep 02 01:59:59 CEST 2016
IPC: C12N 1/20
C12N 1/21
C12N 15/09
C12N 15/52
Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventors: PORTNOY, Daniel A.
PORTMAN, Jonathan L.
Title: METHODS AND COMPOSITIONS FOR MODULATING PRFA MEDIATED VIRULENCE GENE ACTIVATION IN LISTERIA SPP.
Abstract:
Methods of assessing expression of a PrfA mediated expression construct in a Listerial host cell are provided. Aspects of the methods include maintaining a Listerial host cell that includes a PrfA mediated expression construct in a defined medium containing a reducing agent and evaluating expression of a product from the expression construct to assess expression of the PrfA mediated expression construct. Also provided are methods of producing and using a Listerial vaccine, as well as defined media for use in such methods.