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1. (WO2017040813) DETECTION OF GENE LOCI WITH CRISPR ARRAYED REPEATS AND/OR POLYCHROMATIC SINGLE GUIDE RIBONUCLEIC ACIDS

Pub. No.:    WO/2017/040813    International Application No.:    PCT/US2016/049945
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Fri Sep 02 01:59:59 CEST 2016
IPC: C12N 5/071
C12N 5/074
C12N 15/86
C12N 15/63
C12Q 1/68
Applicants: UNIVERSITY OF MASSACHUSETTS
Inventors: PEDERSON, Thoru
MA, Hanhui
LI-CHUNG, Tu
NASERI, Ardalan
XU, Zuoshang
HUISMAN, Maximilaan
YANG, Chunxing
ZHANG, Shaojie
Title: DETECTION OF GENE LOCI WITH CRISPR ARRAYED REPEATS AND/OR POLYCHROMATIC SINGLE GUIDE RIBONUCLEIC ACIDS
Abstract:
A C9orf72 DNA repeat expansion can be detected using a CRISPR Arrayed Repeat Detection System (CARDS). Based upon the compositions and methods supporting this platform primary cell cultures and/or blood cell smears can be tested under conventional clinical diagnostic laboratory conditions to diagnose genetically-based diseases having DNA repeat expansions, including but not limited to ALS. dCas9 constructs are also contemplated as having fluorescent proteins bound to any or all stem loop sequences, wherein detection of a plurality of dCas9 constructs having different colored fluorescent proteins can simultaneously detect at least six (6) different gene target loci.