Search International and National Patent Collections

1. (WO2017040511) COMPOUNDS AND METHODS FOR CRISPR/CAS-BASED GENOME EDITING BY HOMOLOGOUS RECOMBINATION

Pub. No.:    WO/2017/040511    International Application No.:    PCT/US2016/049468
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Wed Aug 31 01:59:59 CEST 2016
IPC: C12N 15/113
C12N 15/63
C12N 9/22
Applicants: AGILENT TECHNOLOGIES, INC.
Inventors: KENNEDY, Andrew
ZEINER, Gusti
ROY, Subhadeep
YUNG, Chong, Wing
Title: COMPOUNDS AND METHODS FOR CRISPR/CAS-BASED GENOME EDITING BY HOMOLOGOUS RECOMBINATION
Abstract:
The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first, guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.