Search International and National Patent Collections

1. (WO2017040477) COMPOSITION AND METHODS FOR DETECTING ADENOSINE MODIFICATIONS

Pub. No.:    WO/2017/040477    International Application No.:    PCT/US2016/049407
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Wed Aug 31 01:59:59 CEST 2016
IPC: A61K 38/48
C12N 9/78
Applicants: THE UNIVERSITY OF CHICAGO
Inventors: HE, Chuan
CHEN, Kai
DAI, Qing
Title: COMPOSITION AND METHODS FOR DETECTING ADENOSINE MODIFICATIONS
Abstract:
The current disclosure relates to methods, compositions and kits for detecting modified adenosine in a target RNA molecule. Aspects relate to a method for detecting modified adenosine in a target ribonucleic acid (RNA) comprising contacting the target RNA with an adenosine deaminase enzyme (adenosine deaminase, RNA-specific) to generate a target RNA with deaminated adenosines and sequencing the target RNA with deaminated adenosines; wherein the modified adenosine is detected when the nucleotide sequence is adenosine.