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1. (WO2017040343) OCHROBACTRUM-MEDIATED TRANSFORMATION OF PLANTS

Pub. No.:    WO/2017/040343    International Application No.:    PCT/US2016/049135
Publication Date: Fri Mar 10 00:59:59 CET 2017 International Filing Date: Sat Aug 27 01:59:59 CEST 2016
IPC: C12N 15/82
C07K 14/195
Applicants: PIONEER HI-BRED INTERNATIONAL, INC.
E. I. DU PONT DE NEMOURS AND COMPANY
Inventors: ANAND, Ajith
BASS, Steven Henry
BERTAIN, Sean M.
CHO, Hyeon-Je
KINNEY, Anthony J
KLEIN, Theodore M.
LASSNER, Michael
MCBRIDE, Kevin E
MOY, York
ROSEN, Barabara Ann Marie
WEI, Jun-Zhi
Title: OCHROBACTRUM-MEDIATED TRANSFORMATION OF PLANTS
Abstract:
Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.