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1. (WO2017040306) NUCLEIC ACID SEQUENCE ANALYSIS FROM SINGLE CELLS
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Pub. No.: WO/2017/040306 International Application No.: PCT/US2016/049046
Publication Date: 09.03.2017 International Filing Date: 26.08.2016
IPC:
C12Q 1/68 (2006.01) ,C12N 15/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
ILLUMINA, INC. [US/US]; 5200 Illumina Way San Diego, California 92122, US
Inventors:
SALATHIA, Neeraj; US
FAN, Jian-Bing; US
KAPER, Fiona; US
CANN, Gordon M.; US
JAMSHIDI, Arash; US
ARAVANIS, Alex; US
Agent:
BAKKER, Jila; US
YODER, Patrick S.; US
FLETCHER, Michael G.; US
SWANSON, Tait R.; US
MANWARE, Robert A.; US
RARIDEN, John M.; US
POWELL, W. Allen; US
SINCLAIR, Steven J., Jr.; US
OSTERHAUS, Matthew G.; US
DOOLEY, Matthew C.; US
KANTOR, Andrew L.; US
GRIER, Jason D.; US
Priority Data:
14/855,20715.09.2015US
62/211,59728.08.2015US
Title (EN) NUCLEIC ACID SEQUENCE ANALYSIS FROM SINGLE CELLS
(FR) ANALYSE DE SÉQUENCES D'ACIDES NUCLÉIQUES PROVENANT DE CELLULES ISOLÉES
Abstract:
(EN) Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).
(FR) L'invention concerne des procédés et des compositions pour l'analyse multiplexe de l'expression de gènes de cellules isolées. Certains procédés et compositions comprennent l'utilisation de gouttelettes et/ou de billes portant des codes à barres uniques tels que des codes à barres moléculaires uniques (UMI).
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)