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1. (WO2017039002) OXIDIZING AGENT FOR 5-HYDROXYMETHYLCYTOSINE AND METHOD FOR ANALYZING 5-HYDROXYMETHYLCYTOSINE
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Pub. No.: WO/2017/039002 International Application No.: PCT/JP2016/075897
Publication Date: 09.03.2017 International Filing Date: 02.09.2016
IPC:
C12N 15/09 (2006.01) ,C09K 3/00 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 33/50 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
09
DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
K
MATERIALS FOR APPLICATIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
3
Materials not provided for elsewhere
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Applicants:
国立大学法人東京大学 THE UNIVERSITY OF TOKYO [JP/JP]; 東京都文京区本郷七丁目3番1号 3-1, Hongo 7-chome, Bunkyo-ku, Tokyo 1138654, JP
Inventors:
岡本 晃充 OKAMOTO Akimitsu; JP
松下 卓 MATSUSHITA Taku; JP
Agent:
平木 祐輔 HIRAKI Yusuke; JP
藤田 節 FUJITA Takashi; JP
漆山 誠一 URUSHIYAMA Seiichi; JP
Priority Data:
2015-17512104.09.2015JP
Title (EN) OXIDIZING AGENT FOR 5-HYDROXYMETHYLCYTOSINE AND METHOD FOR ANALYZING 5-HYDROXYMETHYLCYTOSINE
(FR) AGENT OXYDANT POUR LA 5-HYDROXYMÉTHYLCYTOSINE ET PROCÉDÉ D'ANALYSE DE LA 5-HYDROXYMÉTHYLCYTOSINE
(JA) 5-ヒドロキシメチルシトシン酸化剤及び5-ヒドロキシメチルシトシン解析方法
Abstract:
(EN) The purpose of the present invention is to develop and provide a less expensive and novel oxidizing agent for 5-hydroxymethylcytosine, which can selectively oxidize 5-hydroxymethylcytosine on DNA into 5-formylcytosine while preventing the DNA structure from destabilization and suppressing side reactions, and a method for detecting a demethylation site at a high accuracy in demethylation of DNA with the use of the aforesaid oxidizing agent. Provided is an oxidizing agent for 5-hydroxymethylcytosine that comprises a nitroxyl radical molecule and a copper salt or a copper complex, and/or a nitroxyl radical molecule-copper complex.
(FR) La présente invention vise à mettre au point et à fournir un nouvel agent oxydant, moins coûteux pour la 5-hydroxyméthylcytosine, qui permet d'oxyder sélectivement la 5-hydroxyméthylcytosine sur l'ADN en 5-formylcytosine, tout en empêchant la déstabilisation de la structure d'ADN et en supprimant les réactions secondaires, et un procédé de détection d'un site de déméthylation à une précision élevée dans la déméthylation de l'ADN grâce à l'utilisation de l'agent oxydant susmentionné. L'invention porte sur un agent oxydant pour la 5-hydroxyméthylcytosine qui comprend une molécule radical nitroxyle et un sel de cuivre ou un complexe de cuivre et/ou un complexe de molécule radical de nitroxyle-cuivre.
(JA) DNA構造の不安定化や副反応を抑制し、DNA上の5-ヒドロキシメチルシトシンを選択的に酸化して5-ホルミルシトシンに変換することのできる、安価で新規な5-ヒドロキシメチルシトシン酸化剤、及びその酸化剤を用いてDNA脱メチル化の脱メチル化部位を高い精度で検出する方法を開発し、提供することである。 ニトロキシルラジカル分子及び銅塩又は銅錯体、及び/又はニトロキシルラジカル分子-銅複合体からなる5-ヒドロキシメチルシトシン酸化剤を提供する。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)